Improved glucose electrochemical biosensor by appropriate immobilization of nano-ZnO

被引：69

作者：

Lei, Yang ^{[1
]}

Yan, Xiaoqin ^{[1
]}

Zhao, Jing ^{[1
]}

Liu, Xi ^{[2
]}

Song, Yu ^{[1
]}

Luo, Ning ^{[1
]}

Zhang, Yue ^{[1
,2
]}

机构：

[1] Univ Sci & Technol Beijing, Sch Mat Sci & Engn, State Key Lab Adv Met & Mat, Beijing 100083, Peoples R China

[2] Univ Sci & Technol Beijing, Key Lab New Energy Mat & Technol, Beijing 100083, Peoples R China

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2011年 / 82卷 / 01期

基金：

中国国家自然科学基金;

关键词：

ZnO nanorod; Biosensor; Glucose oxidase; Electrochemistry; AMPEROMETRIC BIOSENSOR; ELECTRODE; OXIDASE; FILM; NANOPARTICLES; NANOTUBES; ENZYME; ARRAY; ACID;

D O I：

10.1016/j.colsurfb.2010.08.034

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We constructed the transferred ZnO biosensor and the grown ZnO biosensor by two different nano ZnO immobilization approaches And the influence of different assembly processes on the biosensor performance has been systematically investigated and compared An enhanced sensitivity of the grown ZnO biosensor is found to be 52% higher than that of the transferred ZnO biosensor Correspondingly the other properties are also better in the grown ZnO biosensor including the response time the detection limit and the linear range These results are well consistent with the fact that more glucose oxidase is Immobilized on the well aligned ZnO arrays which have higher specific surface area and more direct electron communication path in the grown sensor than the randomly distributed and stacked ZnO nanorods in the transferred sensor The nano ZnO grown directly has been demonstrated more desirable for enzymatic immobilization and signal transduction in the high performance biosensors (C) 2010 Elsevier B.V. All rights reserved

引用

页码：168 / 172

页数：5

共 29 条

[1] Covalent attachment of osmium complexes to glucose oxidase and the application of the resulting modified enzyme in an enzyme switch responsive to glucose [J].