The use of gene therapy tools in reproductive immunology research

被引：5

作者：

Zenclussen, AC ^{[1
]}

Zenclussen, ML ^{[1
]}

Ritter, T ^{[1
]}

Volk, HD ^{[1
]}

机构：

[1] Med Univ, Charite, BMFZ, Inst Med Immunol, D-13353 Berlin, Germany

来源：

CURRENT GENE THERAPY | 2005年 / 5卷 / 05期

关键词：

Gene Therapy; reproductive immunology; HO-1; fetal-maternal interface;

D O I：

10.2174/156652305774329276

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Mammalian pregnancy is a complex phenomenon allowing the maternal immune system to support its allogeneic fetus, while still being effective against pathogens. Gene therapy approaches have the potential to treat devastating inherited diseases for which there is a little hope of finding a conventional cure. In reproductive medicine, experimental trials have been made so far only for correcting gene defects in utero. The use of gene therapy for improving pregnancy-rate success or avoiding pregnancy-related diseases i.e. miscarriage or pre-eclampsia, remains a very distant goal with unresolved moral and ethical aspects. However, gene therapy may help determining the role of several genes in supporting fetal growth and/or avoiding its rejection experimentally and might further help to identify new targets of intervention. Gene therapy strategies to avoid fetal rejection may include the transfer and expression of cyto-protective molecules locally at the fetal-placental interface. In addition, the ex-vivo genetic modification of immune cells for tolerance induction is a novel and tempting approach. In this regard, we have confirmed the role of the cyto-protective and immunomodulatory molecule Heme Oxygenase-1 (HO-1), by treating animals undergoing abortion with an adenovirus coding for HO-1. Since the sole application of a control vector did not provoke deleterious effects in pregnancy outcome, we propose the use of experimental gene therapy for unveiling molecular and cellular pathways leading to pregnancy success.

引用

页码：459 / 466

页数：8

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