PI3K is a key molecule in the Nrf2-mediated regulation of antioxidative proteins by hemin in human neuroblastoma cells

被引：292

作者：

Nakaso, K ^{[1
]}

Yano, H ^{[1
]}

Fukuhara, Y ^{[1
]}

Takeshima, T ^{[1
]}

Wada-Isoe, K ^{[1
]}

Nakashima, K ^{[1
]}

机构：

[1] Tottori Univ, Fac Med, Inst Neurol Sci, Dept Neurol, Yonago, Tottori 6838504, Japan

来源：

FEBS LETTERS | 2003年 / 546卷 / 2-3期

关键词：

phosphatidyl inositol 3-kinase; NF-E2-related factor 2; antioxidant responsive element; heme oxygenase-1; thioredoxin; peroxiredoxin;

D O I：

10.1016/S0014-5793(03)00517-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Oxidative stress and ferrous metabolism are important in the pathogenesis in Parkinson's disease. In dopaminergic neurons, several stress proteins are upregulated under oxidative stress. To clarify this mechanism, we investigated hemin-related signal transduction and the induction of oxidative stress-related proteins in SH-SY5Y cells. We identified phosphatidylinositol 3-kinase (PI3K) and Nrf2 as important molecules in the induction of heme oxygenase-1, thioredoxin, and peroxiredoxin-I. PI3K-related signal controlled Nrf2 activation, and consequently, PI3k inhibitors blocked the nuclear translocation of Nrf2 and induction of stress proteins. These observations suggest that PI3K and Nrf2 are key molecules in maintaining suitable conditions under oxidative stress and ferrous metabolism. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：181 / 184

页数：4

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