Determination of the molecular mass of amylose

Normally the reliable determination of the molecular mass of amylose is a very tedious procedure requiring several days of sample preparation to remove contaminating amylopectin. In the method presented the detection of amylose is based on its selective detection by post-column colourization after size-separation chromatographic separation. The quantification of amylose is based on totally linear synthetic amylose thus targeting the analysis on the most important quality of amylose, long linear chains. The molecular mass of amylose, which was the main target could be analyzed by very simple sample preparation. (C) 1998 Published by Elsevier Science BN. All rights reserved.