Fluorescence microscopy

被引：1182

作者：

Lichtman, JW

Conchello, JA

机构：

[1] Harvard Univ, Dept Mol & Cell Biol, Cambridge, MA 02138 USA

[2] Oklahoma Med Res Fdn, Oklahoma City, OK 73104 USA

[3] Univ Oklahoma, Biomed Engn Program, Norman, OK 73019 USA

来源：

NATURE METHODS | 2005年 / 2卷 / 12期

关键词：

D O I：

10.1038/NMETH817

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Although fluorescence microscopy permeates all of cell and molecular biology, most biologists have little experience with the underlying photophysical phenomena. Understanding the principles underlying fluorescence microscopy is useful when attempting to solve imaging problems. Additionally, fluorescence microscopy is in a state of rapid evolution, with new techniques, probes and equipment appearing almost daily. Familiarity with fluorescence is a prerequisite for taking advantage of many of these developments. This review attempts to provide a framework for understanding excitation of and emission by fluorophores, the way fluorescence microscopes work, and some of the ways fluorescence can be optimized.

引用

页码：910 / 919

页数：10

共 36 条

[21]

Mason W. T., 1993, FLUORESCENT LUMINESC

[22] Photo-induced peptide cleavage in the green-to-red conversion of a fluorescent protein [J].