Trapping parasite secretory proteins in baker's yeast

被引：8

作者：

Nene, V ^{[1
]}

Bishop, R ^{[1
]}

机构：

[1] Int Livestock Res Inst, Nairobi, Kenya

来源：

TRENDS IN PARASITOLOGY | 2001年 / 17卷 / 09期

关键词：

D O I：

10.1016/S1471-4922(01)02029-3

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Because the function of signal sequences has been conserved during evolution it has been possible to develop both bioinformatics resources to identify them and techniques to clone genes that encode secretory proteins. The latter entail insertion of heterologous signals upstream of signal peptide deleted reporter genes. We discuss the advantages of using Saccharomyces cerevisiae for signal sequence trap technology. The yeast protein-translocation system appears to be less discriminating than that of higher eukaryotes-for example, a Theileria parva cysteine protease gene containing a recessed, nonclassical signal allows access to the secretory pathway-and yeast technology could have general application in studying elements of parasite protein trafficking.

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收藏

页码：407 / 409

页数：3

相关论文

共 19 条

[1] A new signal sequence trap using alkaline phosphatase as a reporter [J].

Chen, HC ;

Leder, P .

NUCLEIC ACIDS RESEARCH, 1999, 27 (04) :1219-1222

[2] Characterization of a secretory type Theileria parva glutaredoxin homologue identified by novel screening procedure [J].

Ebel, T ;

Middleton, JFS ;

Frisch, A ;

Lipp, J .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (05) :3042-3048

[3] Protein trafficking in malaria-infected erythrocytes [J].

Foley, M ;

Tilley, L .

INTERNATIONAL JOURNAL FOR PARASITOLOGY, 1998, 28 (11) :1671-1680

[4] Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum [J].

Gardner, MJ ;

Tettelin, H ;

Carucci, DJ ;

Cummings, LM ;

Aravind, L ;

Koonin, EV ;

Shallom, S ;

Mason, T ;

Yu, K ;

Fujii, C ;

Pederson, J ;

Shen, K ;

Jing, JP ;

Aston, C ;

Lai, ZW ;

Schwartz, DC ;

Pertea, M ;

Salzberg, S ;

Zhou, LX ;

Sutton, GG ;

Clayton, R ;

White, O ;

Smith, HO ;

Fraser, CM ;

Adams, MD ;

Venter, JC ;

Hoffman, SL .

SCIENCE, 1998, 282 (5391) :1126-1132

[5]

Goo JH, 1999, MOL CELLS, V9, P564

[6] Regulation of protein biogenesis at the endoplasmic reticulum membrane [J].

Hegde, RS ;

Lingappa, VR .

TRENDS IN CELL BIOLOGY, 1999, 9 (04) :132-137

[7] Molecular cloning of a novel T cell-directed CC chemokine expressed in thymus by signal sequence trap using Epstein-Barr virus vector [J].

Imai, T ;

Yoshida, T ;

Baba, M ;

Nishimura, M ;

Kakizaki, M ;

Yoshie, O .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (35) :21514-21521

[8] A genetic selection for isolating cDNAs encoding secreted proteins [J].

Jacobs, KA ;

CollinsRacie, LA ;

Colbert, M ;

Duckett, M ;

GoldenFleet, M ;

Kelleher, K ;

Kriz, R ;

LaVallie, ER ;

Merberg, D ;

Spaulding, V ;

Stover, J ;

Williamson, MJ ;

McCoy, JM .

GENE, 1997, 198 (1-2) :289-296

[9] MANY RANDOM SEQUENCES FUNCTIONALLY REPLACE THE SECRETION SIGNAL SEQUENCE OF YEAST INVERTASE [J].

KAISER, CA ;

PREUSS, D ;

GRISAFI, P ;

BOTSTEIN, D .

SCIENCE, 1987, 235 (4786) :312-317

[10] Selection for genes encoding secreted proteins and receptors [J].

Klein, RD ;

Gu, QM ;

Goddard, A ;

Rosenthal, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (14) :7108-7113