Serum chloride optical sensors based on dynamic quenching of the fluorescence of photo-immobilized lucigenin

被引：29

作者：

Huber, C ^{[1
]}

Krause, C ^{[1
]}

Werner, T ^{[1
]}

Wolfbeis, OS ^{[1
]}

机构：

[1] Univ Regensburg, Inst Analyt Chem Chemo & Biosensors, D-93040 Regensburg, Germany

来源：

MICROCHIMICA ACTA | 2003年 / 142卷 / 04期

关键词：

chloride sensor; fluorescence; quenching; lucigenin; photo-immobilization;

D O I：

10.1007/s00604-003-0034-0

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This article describes the preparation and performance of an optical sensor for continuous measurement of chloride at extracellular (serum) levels (20-200 mM). The sensor is based on dynamic quenching of the fluorescence of lucigenin which was photo-immobilized on a hydrogel. Quenching occurs via a collisional mechanism, and the decrease in fluorescence intensity on exposure to 100 mM chloride typically is -60%. It allows the determination of chloride in the 1 to 200 mM concentration range, with a precision of +/-3 mM at 120 mM. Bromide, iodide and salicylate act as interferents, while the effect of pH and oxygen is negligibly small. The sensor displays strong fluorescence intensity, excellent reproducibility, long-term stability, response times in the order of 2-5 min, and is used in a commercial serum and blood chloride analyzer.

引用

页码：245 / 253

页数：9

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