Mutasynthesis of aureonitrile: An aureothin derivative with significantly improved cytostatic effect

被引：46

作者：

Ziehl, M ^{[1
]}

He, J ^{[1
]}

Dahse, HM ^{[1
]}

Hertweck, C ^{[1
]}

机构：

[1] Hans Knoell Inst Nat Prod Res, D-07745 Jena, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2005年 / 44卷 / 08期

关键词：

antitumor agents; mutasynthesis; natural products; polyketides;

D O I：

10.1002/anie.200461990

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Tapping nature's biosynthetic potential: Formation of the rare p-nitro benzoate precursor of aureothin, a mixed polyketide metabolite of Streptomyces thioluteus, requires two key enzymes: a p-amino benzoate synthase and an N-oxygenase. Feeding of surrogates to engineered block mutants (e.g. S. lividans ZX1::pHJ97) reveals that the iterative modular aureothin polyketide also accepts p-cyano benzoate as the starter, which is subsequently processed to the nitrile analogue of aureothin, aureonitrile, which has improved cytostatic properties. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：1202 / 1205

页数：4

共 28 条

[1] A role for pabAB, a p-aminobenzoate synthase gene of Streptomyces venezuelae ISP5230, in chloramphenicol biosynthesis [J].