Single channel properties of lysenin measured in artificial lipid bilayers and their applications to biomolecule detection

被引：12

作者：

Aoki, Takaaki ^{[1
]}

Hirano, Minako ^{[2
]}

Takeuchi, Yuko ^{[1
]}

Kobayashi, Toshihide ^{[3
]}

Yanagida, Toshio ^{[1
,2
]}

Ide, Toru ^{[1
,4
]}

机构：

[1] Osaka Univ, Grad Sch Frontier Biosci, Network Ctr Mol & Syst Life Sci, Suita, Osaka 5650871, Japan

[2] Osaka Univ, Grad Sch Frontier Biosci, Lab Nanobiol, Suita, Osaka 5650871, Japan

[3] RIKEN, Lipid Biol Lab, Saitama, Japan

[4] RIKEN, Mol Informat Life Sci Res Grp, Saitama, Japan

来源：

PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES | 2010年 / 86卷 / 09期

关键词：

hemolytic toxin; patch clamp; single channel current; current inhibition; EISENIA-FOETIDA; SPHINGOMYELIN; MEMBRANES; PROTEIN; TOXIN; BINDING;

D O I：

10.2183/pjab.86.920

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Single channel currents of lysenin were measured using artificial lipid bilayers formed on a glass micropipette tip. The single channel conductance for KCl, NaCl, CaCl2, and Trimethylammonium-Cl were 474 +/- 87, 537 +/- 66, 210 +/- 14, and 274 +/- 10 ps, respectively, while the permeability ratio P-Na/P-Cl was 5.8. By adding poly(deoxy adenine) or poly(L-lysine) to one side of the bilayer, channel currents were influenced when membrane voltages were applied to pass the charged molecules through the channel pores. Current inhibition process was concentration-dependent with applied DNA. As the current fluctuations of a-hemolysin channels is often cited as the detector in a molecular sensor, these results suggest that by monitoring channel current changes, the lysenin channel has possibilities to detect interactions between it and certain biomolecules by its current fluctuations.

引用

页码：920 / 925

页数：6

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