Herpes simplex virus type 2 UL24 gene is a virulence determinant in murine and guinea pig disease models

被引：31

作者：

Blakeney, S

Kowalski, J

Tummolo, D

DeStefano, J

Cooper, D

Guo, M

Gangolli, S

Long, D

Zamb, T

Natuk, RJ

Visalli, RJ

机构：

[1] Indiana Univ Purdue Univ, Dept Biol, Ft Wayne, IN 46805 USA

[2] Wyeth Vaccines Res, Pearl River, NY 10965 USA

来源：

JOURNAL OF VIROLOGY | 2005年 / 79卷 / 16期

关键词：

D O I：

10.1128/JVI.79.16.10498-10506.2005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A herpes simplex virus type 2 (HSV-2) UL24 beta-glucuronidase (UL24-beta gluc) insertion mutant was derived from HSV-2 strain 186 via standard marker transfer techniques. Cell monolayers infected with UL24-beta gluc yielded cytopathic effect with syncytium formation. UL24-beta gluc replicated to wild-type viral titers in three different cell lines. UL24-beta gluc was not virulent after intravaginal inoculation of BALB/c mice in that all inoculated animals survived doses up to 400 times the 50% lethal dose (LD50) of the parental virus. Furthermore, few UL24-beta gluc-inoculated mice developed any vaginal lesions. Intravaginal inoculation of guinea pigs with UL24-beta gluc at a dose equivalent to the LD50 of parental virus (approximate to 5 x 10(3) PFU) was not lethal (10/10 animals survived). Although genital lesions developed in some UL24-beta gluc-inoculated guinea pigs, both the overall number of lesions and the severity of disease were far less than that observed for animals infected with parental strain 186.

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收藏

页码：10498 / 10506

页数：9

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