Lipid transfer proteins from fruit:: Cloning, expression and quantification

被引:44
作者
Zuidmeer, L
van Leeuwen, WA
Budde, IK
Cornelissen, J
Bulder, I
Rafalska, I
Besolí, NT
Akkerdaas, JH
Asero, R
Rivas, MF
Mancebo, EG
van Ree, R
机构
[1] CLB Sanquin, Dept Immunopathol, NL-1066 CX Amsterdam, Netherlands
[2] Osped Caduti Bollatesi, Ambulatorio Allergol, Bollate, MI, Italy
[3] Fdn Hosp Alcorcon, Allergy Unit, Madrid, Spain
关键词
food allergy; apple allergy; lipid transfer protein; recombinant allergens; measurement;
D O I
10.1159/000086420
中图分类号
R392 [医学免疫学];
学科分类号
100102 ;
摘要
Background: Lipid transfer proteins (LTP) are stable, potentially life-threatening allergens in fruits and many other vegetable foods. The aim of this study was to clone and express recombinant apple LTP (Mal d 3), as has previously been done for peach LTP (Pru p 3) and set up quantitative tests for measuring fruit LTPs. Methods: cDNA for Mal d 3 and Pru p 3 was cloned, expressed in the yeast Pichia pastoris and the resulting proteins were purified via cation exchange chromatography. The immune reactivity of rMal d 3 was compared to nMal d 3 by RAST (inhibition), immunoblotting and basophil histamine release testing. To obtain monoclonal and monospecific polyclonal antibodies, mice and rabbits were immunized with purified nMal d 3. Results: The deduced amino acid sequence of Mal d 3 was identical to the published sequence, Pru p 3 differed at two positions (S9A and S76H). The rMal d 3 had an IgE-binding potency and biological activity close to its natural counterpart. One sandwich ELISA selectively detecting apple LTP and another cross-reactive with cherry, nectarine and hazelnut LTP were developed. In addition, a competitive RIA was developed with polyclonal rabbit antiserum and labeled nMal d 3. Conclusion: rMal d 3 (as shown before for rPru p 3) may be a useful tool for application in component-resolved diagnosis of food allergy. Assays for the measurement of LTP will increase the traceability of this potentially dangerous allergen. Copyright (C) 2005 S. Karger AG, Basel.
引用
收藏
页码:273 / 281
页数:9
相关论文
共 47 条
[1]   IMMUNOGLOBULIN-E ANTIBODIES THAT CROSSREACT WITH VEGETABLE FOODS, POLLEN, AND HYMENOPTERA VENOM [J].
AALBERSE, RC ;
KOSHTE, V ;
CLEMENS, JGJ .
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1981, 68 (05) :356-364
[2]   Analysis of the heat stability of lipid transfer protein from apple [J].
Asero, R ;
Mistrello, G ;
Roncarolo, D ;
Amato, S ;
Falagiani, P .
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 2003, 112 (05) :1009-1011
[3]   Lipid transfer protein: A pan-allergen in plant-derived foods that is highly resistant to pepsin digestion [J].
Asero, R ;
Mistrello, G ;
Roncarolo, D ;
de Vries, SC ;
Gautier, MF ;
Ciurana, LF ;
Verbeek, E ;
Mohammadi, T ;
Knul-Brettlova, V ;
Akkerdaas, JH ;
Bulder, I ;
Aalberse, RC ;
van Ree, R .
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, 2000, 122 (01) :20-32
[4]  
ASTALDI GCB, 1980, J IMMUNOL, V125, P1411
[5]   Celery allergy confirmed by double-blind, placebo-controlled food challenge:: A clinical study in 32 subjects with a history of adverse reactions to celery root [J].
Ballmer-Weber, BK ;
Vieths, S ;
Lüttkopf, D ;
Heuschmann, P ;
Wüthrich, B .
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 2000, 106 (02) :373-378
[6]   Component-resolved diagnosis with recombinant allergens in patients with cherry allergy [J].
Ballmer-Weber, BK ;
Scheurer, S ;
Fritsche, P ;
Enrique, E ;
Cistero-Bahima, A ;
Haase, T ;
Wüthrich, B .
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 2002, 110 (01) :167-173
[7]   EXTRACTION AND PROPERTIES OF APPLE ALLERGENS [J].
BJORKSTEN, F ;
HALMEPURO, L ;
HANNUKSELA, M ;
LAHTI, A .
ALLERGY, 1980, 35 (08) :671-677
[8]   Allergy to jackfruit: a novel example of Bet v 1-related food allergy [J].
Bolhaar, STHP ;
van Ree, R ;
Bruijnzeel-Koomen, CAFM ;
Knulst, AC ;
Zuidmeer, L .
ALLERGY, 2004, 59 (11) :1187-1192
[9]   CROSS-REACTIVITY AMONG BIRCH POLLEN, VEGETABLES AND FRUITS AS DETECTED BY IGE ANTIBODIES IS DUE TO AT LEAST 3 DISTINCT CROSS-REACTIVE STRUCTURES [J].
CALKHOVEN, PG ;
AALBERS, M ;
KOSHTE, VL ;
POS, O ;
OEI, HD ;
AALBERSE, RC .
ALLERGY, 1987, 42 (05) :382-390
[10]   The abundant 31-kilodalton banana pulp protein is homologous to class-III acidic chitinases [J].
Clendennen, SK ;
López-Gómez, R ;
Gómez-Lim, M ;
Arntzen, CJ ;
May, GD .
PHYTOCHEMISTRY, 1998, 47 (04) :613-619