On-line dialysis-SPE-CE of acidic drugs in biological samples

被引：39

作者：

Veraart, JR ^{[1
]}

Groot, MCE ^{[1
]}

Gooijer, C ^{[1
]}

Lingeman, H ^{[1
]}

Velthorst, NH ^{[1
]}

Brinkman, UAT ^{[1
]}

机构：

[1] Vrije Univ Amsterdam, Dept Analyt Chem, NL-1081 HV Amsterdam, Netherlands

来源：

ANALYST | 1999年 / 124卷 / 02期

关键词：

D O I：

10.1039/a807636g

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A fully automated method is presented for the determination of acidic drugs in urine and serum using on-line dialysis-solid-phase extraction (SPE)-capillary electrophoresis (CE) with UV detection. With non-steroidal anti-inflammatory drugs (NSAIDs) as test compounds, detection limits in the biological samples were 0.05-1.0 mu g ml(-1). Calibration plots were linear over two orders of magnitude and the within-day and between-day repeatability were better than 10%. The CE capillary and SPE column were used for over 500 analyses; the dialysis membrane was replaced after 250 analyses. A general protocol for dialysis-SPE-CE which can be used for amphoteric and acidic drugs was devised. The present results show that this protocol has general validity and can be recommended for future work on other classes of drugs.

引用

页码：115 / 118

页数：4

共 11 条

[1]

[Anonymous], J CHROMATOGR B

[2]

DANATO MG, 1994, J PHARM BIOMED ANAL, V12, P21

[3]

DANATO MG, 1993, J PHARM BIOMED ANAL, V11, P197

[4] USE OF CYCLODEXTRINS IN CAPILLARY ELECTROPHORESIS FOR THE CHIRAL RESOLUTION OF SOME 2-ARYLPROPIONIC ACID NONSTEROIDAL ANTIINFLAMMATORY DRUGS [J].

FANALI, S ;

ATURKI, Z .

JOURNAL OF CHROMATOGRAPHY A, 1995, 694 (01) :297-305

[5] PRACTICAL ASPECTS IN CHIRAL SEPARATION OF PHARMACEUTICALS BY CAPILLARY ELECTROPHORESIS .2. QUANTITATIVE SEPARATION OF NAPROXEN ENANTIOMERS [J].

GUTTMAN, A ;

COOKE, N .

JOURNAL OF CHROMATOGRAPHY A, 1994, 685 (01) :155-159

[6] IDENTIFICATION AND SIMULTANEOUS DETERMINATION OF NON-STEROIDAL ANTI-INFLAMMATORY DRUGS USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

LAPICQUE, F ;

NETTER, P ;

BANNWARTH, B ;

TRECHOT, P ;

GILLET, P ;

LAMBERT, H ;

ROYER, RJ .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1989, 496 (02) :301-320

[7] SEPARATION OF 15 NONSTEROIDAL ANTIINFLAMMATORY DRUGS USING MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY [J].

MABOUNDOU, CW ;

PAINTAUD, G ;

BERARD, M ;

BECHTEL, PR .

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS, 1994, 657 (01) :173-183

[8] DETERMINATION OF NAPROXEN IN SERUM BY CAPILLARY ELECTROPHORESIS WITH ULTRAVIOLET ABSORBENCY AND LASER-INDUCED FLUORESCENCE DETECTION [J].

SOINI, H ;

NOVOTNY, MV ;

RIEKKOLA, ML .

JOURNAL OF MICROCOLUMN SEPARATIONS, 1992, 4 (04) :313-318

[9] AUTOMATED PREPARATION OF BIOLOGICAL SAMPLES PRIOR TO HIGH-PRESSURE LIQUID-CHROMATOGRAPHY .1. THE USE OF DIALYSIS FOR DEPROTEINIZING SERUM FOR AMINO-ACID-ANALYSIS [J].

TURNELL, DC ;

COOPER, JDH .

JOURNAL OF AUTOMATIC CHEMISTRY, 1985, 7 (04) :177-180

[10] On-line dialysis solid-phase extraction coupled to capillary electrophoresis [J].

Veraart, JR ;

van Hekezen, J ;

Groot, MCE ;

Gooijer, C ;

Lingeman, H ;

Velthorst, NH ;

Brinkman, UAT .

ELECTROPHORESIS, 1998, 19 (16-17) :2944-2949

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