PRACTICAL ASPECTS IN CHIRAL SEPARATION OF PHARMACEUTICALS BY CAPILLARY ELECTROPHORESIS .2. QUANTITATIVE SEPARATION OF NAPROXEN ENANTIOMERS

In recent years, there has been considerable activity in the separation and characterization of optically active molecules. In this paper we report a new, improved and automated electrophoretic method for the separation of enantiomers in the form of high-performance capillary gel electrophoresis using hydroxypropyl-beta-cyclodextrin as a chiral selector. Rapid, efficient separation of naproxen enantiomers is shown with very low detection limits and excellent detection linearity. The intra- and inter-day as well as intra- and inter-column migration time reproducibility was less than 2% R.S.D. Trace level enantiomeric contamination determination is also shown.