Integrated electrochemical sensor array for on-line monitoring of yeast fermentations

被引:40
作者
Krommenhoek, E. E.
Gardeniers, J. G. E.
Bomer, J. G.
Li, X.
Ottens, M.
van Dedem, G. W. K.
Van Leeuwen, M.
van Gulik, W. M.
van der Wielen, L. A. M.
Heijnen, J. J.
van den Berg, A.
机构
[1] Univ Twente, MESA, Inst Nanotechnol, NL-7500 AE Enschede, Netherlands
[2] Delft Univ Technol, Dept Biotechnol, Delft, Netherlands
关键词
D O I
10.1021/ac0623078
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
This paper describes the design, modeling, and experimental characterization of an electrochemical sensor array for on-line monitoring of fermentor conditions in both miniaturized cell assays and in industrial scale fertnentations. The viable biomass concentration is determined from impedance spectroscopy. As a miniaturized electrode configuration with high cell constant is applied, the spectral conductivity variation is monitored instead of the permittivity variation. The dissolved oxygen concentration is monitored amperometrically using an ultra-microelectrode array, which is shown to have negligible flow dependence. pH is monitored using an ion-sensitive field effect transistor (ISFET), and a platinum thermistor is included for temperature measurements. All sensors were shown to be sufficiently accurate within the range relevant to yeast fermentations. The sensor array is shown to be very stable and durable and withstands steam-sterilization.
引用
收藏
页码:5567 / 5573
页数:7
相关论文
共 45 条
[1]   Characterization of biological cells by dielectric spectroscopy [J].
Asami, K .
JOURNAL OF NON-CRYSTALLINE SOLIDS, 2002, 305 (1-3) :268-277
[2]   Dielectric behavior of non-spherical cells in culture [J].
Asami, K ;
Yonezawa, T .
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1995, 1245 (03) :317-324
[3]   Dielectric behavior of budding yeast in cell separation [J].
Asami, K ;
Gheorghiu, E ;
Yonezawa, T .
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1998, 1381 (02) :234-240
[4]   DIELECTRIC ANALYSIS OF YEAST-CELL GROWTH [J].
ASAMI, K ;
YONEZAWA, T .
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1995, 1245 (01) :99-105
[5]   Long-term monitoring of bacteria undergoing programmed population control in a microchemostat [J].
Balagaddé, FK ;
You, LC ;
Hansen, CL ;
Arnold, FH ;
Quake, SR .
SCIENCE, 2005, 309 (5731) :137-140
[6]  
BARD AJ, 1980, ELECTROCHEMICAL METH
[7]   Thirty years of ISFETOLOGY - What happened in the past 30 years and what may happen in the next 30 years [J].
Bergveld, P .
SENSORS AND ACTUATORS B-CHEMICAL, 2003, 88 (01) :1-20
[9]  
BERGVELD P, 1988, ANAL BIOMED APPL ION
[10]  
BRETT CMA, 1983, PRINCIPLES METHODS A