Human TRIM71 and its nematode homologue are targets of let-7 MicroRNA and its zebrafish orthologue is essential for development

被引:68
作者
Lin, You-Chin
Hsieh, Li-Ching
Kuo, Ming-Wei
Yu, John
Kuo, Huan-Hsien
Lo, Wan-Lin
Lin, Ruey-Jen
Yu, Alice L.
Li, Wen-Hsiung [1 ]
机构
[1] Acad Sinica, Genom Res Ctr, Taipei 115, Taiwan
[2] Acad Sinica, Inst Informat Sci, Taipei 115, Taiwan
[3] Acad Sinica, Inst Cellular & Organism Biol, Taipei 115, Taiwan
[4] Univ Calif San Diego, Dept Pediat Hematol Oncol, San Diego, CA 92103 USA
[5] Univ Chicago, Dept Ecol & Evolut, Chicago, IL 60637 USA
关键词
TRIM71; lin-41; let-7; microRNA target;
D O I
10.1093/molbev/msm195
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Animal microRNAs (miRNAs) are short RNAs that function as posttranscriptional regulators of gene expression by binding to the target mRNAs. Noting that some miRNAs are highly conserved in evolution, we explored the possibility of evolutionary conservation of their targets. We identified human orthologues of experimentally verified let-7 miRNA target genes in Caenorhabditis elegans and used the luciferase reporter system to examine whether these human genes are still the targets of let-7 miRNA. We found that in some cases, the miRNA-target relationship has indeed been conserved in human. Interestingly, human TRIM71, an orthologue of C. elegans let-7-target lin-41 gene, can be repressed by hsa-let-7a and hsa-let-7c. This repression was abolished when both predicted let-7 target sites of TRIM71 were mutated. Moreover, the zebrafish lin-41 orthologue was also repressed by let-7 to a similar degree as was TRIM71. When the expression of zebrafish lin-41 orthologue was silenced by microinjection of RNA interference or morpholino into zebrafish zygotes, retarded embryonic development was observed, providing direct evidence for an essential role of lin-41 in zebrafish development. Taken together, our results suggest that the regulation of TRIM71 expression by let-7 has been evolutionarily conserved and that TRIM71 likely plays an important role in development.
引用
收藏
页码:2525 / 2534
页数:10
相关论文
共 61 条
[1]   BASIC LOCAL ALIGNMENT SEARCH TOOL [J].
ALTSCHUL, SF ;
GISH, W ;
MILLER, W ;
MYERS, EW ;
LIPMAN, DJ .
JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410
[2]   The functions of animal microRNAs [J].
Ambros, V .
NATURE, 2004, 431 (7006) :350-355
[3]  
Antebi A, 2000, GENE DEV, V14, P1512
[4]  
Antebi A, 1998, DEVELOPMENT, V125, P1191
[5]   A fork head/HNF-3 homolog expressed in the pharynx and intestine of the Caenorhabditis elegans embryo [J].
Azzaria, M ;
Goszczynski, B ;
Chung, MA ;
Kalb, JM ;
McGhee, JD .
DEVELOPMENTAL BIOLOGY, 1996, 178 (02) :289-303
[6]   Regulation by let-7 and lin-4 miRNAs results in target mRNA degradation [J].
Bagga, S ;
Bracht, J ;
Hunter, S ;
Massirer, K ;
Holtz, J ;
Eachus, R ;
Pasquinelli, AE .
CELL, 2005, 122 (04) :553-563
[7]   MicroRNAs: Genomics, biogenesis, mechanism, and function (Reprinted from Cell, vol 116, pg 281-297, 2004) [J].
Bartel, David P. .
CELL, 2007, 131 (04) :11-29
[8]   Identification of a human nuclear receptor defines a new signaling pathway for CYP3A induction [J].
Bertilsson, G ;
Heidrich, J ;
Svensson, K ;
Åsman, M ;
Jendeberg, L ;
Sydow-Bäckman, M ;
Ohlsson, R ;
Postlind, H ;
Blomquist, P ;
Berkenstam, A .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (21) :12208-12213
[9]   SXR, a novel steroid and xenobiotic-sensing nuclear receptor [J].
Blumberg, B ;
Sabbagh, W ;
Juguilon, H ;
Bolado, J ;
van Meter, CM ;
Ono, ES ;
Evans, RM .
GENES & DEVELOPMENT, 1998, 12 (20) :3195-3205
[10]   MicroRNAs regulate the expression of the alternative splicing factor nPTB during muscle development [J].
Boutz, Paul L. ;
Chawla, Geetanjali ;
Stoilov, Peter ;
Black, Douglas L. .
GENES & DEVELOPMENT, 2007, 21 (01) :71-84