Energy transfer between CdSe/ZnS Core/Shell quantum dots and fluorescent proteins

被引：36

作者：

Hering, Vitor R. ^{[1
]}

Gibson, Gary

Schumacher, Robert I.

Faljoni-Alario, Adelaide

Politi, Mario J.

机构：

[1] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05513970 Sao Paulo, Brazil

[2] Hewlett Packard Labs, Palo Alto, CA 94304 USA

来源：

BIOCONJUGATE CHEMISTRY | 2007年 / 18卷 / 06期

关键词：

D O I：

10.1021/bc700147j

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Fluorescent proteins from the green fluorescent protein (GFP) family interact strongly with CdSe/ZnS quantum dots. Photoluminescence of GFP5 is suppressed by red-emitting CdSe/ZnS quantum dots with high efficiency in a pH-dependent manner. The elevated degree of quenching, around 90%, makes it difficult to analyze the remaining signal, and it is not clear yet whether FRET is the reason behind the quenching. When the donor is a green-emitting CdSe/ZnS quantum dot and the acceptor is the HcRed 1 protein, it is possible to detect quenching of the donor and sensitized emission from the acceptor. It was verified that the sensitized emission has the low anisotropy characteristic of FRET. The present characterization identifies donor-acceptor pairs formed by fluorescent proteins and CdSe/ZnS quantum dots that are suitable for the exploration of cellular events. These donor-acceptor pairs take advantage of the exceptional photochemical properties of quantum dots allied with the unique ability of fluorescent proteins to act as gene-based fluorescent probes.

引用

页码：1705 / 1708

页数：4

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