A new laccase biosensor for polyphenols determination

被引:78
作者
Gomes, SASS [1 ]
Rebelo, MJF [1 ]
机构
[1] Univ Lisbon, Fac Ciencias, CECUL, P-1749016 Lisbon, Portugal
来源
SENSORS | 2003年 / 3卷 / 06期
关键词
biosensor; laccase; polyphenol; (+)-catechin; caffeic acid;
D O I
10.3390/s30600166
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The relevance of polyphenols in human health is a well known fact. Prompted by that, very intensive research has been directed to get a method to detect them, which will improve the current ones. Laccase (p-diphenol:dioxygen oxidoreductase, EC 1.10.3.2) is a multi-copper oxidase, which couples catalytic oxidation of phenolic substrates with four electron reduction of dioxygen to water [1]. A maximum catalytic response in oxygenated electrolyte was observed between 4.5 and 5.5 [2], while for pH > 6.9 the laccase was found to be inactive [3]. We prepared a biosensor with laccase immobilised on a polyether sulphone membrane, at pH 4.5, which was applied at Universal Sensors base electrode. Reduction of the product of oxidation of several polyphenols, catalysed by laccase, was done at a potential for which the polyphenol of interest was found to respond. Reduction of catechol was found to occur at a potential of 200mV, which is often referred to in the literature for polyphenolic biosensors. However other polyphenols did not respond at that potential. It was observed that (+)- catechin produced a very large cathodic current when +100mV were applied to the laccase biosensor, both in aqueous acetate and 12% ethanol acetate buffer, whereas caffeic acid responded at 50mV. Other polyphenols tested were gallic acid, malvidin, quercetin, rutin, trans-resveratrol.
引用
收藏
页码:166 / 175
页数:10
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