Large volume sample stacking capillary electrophoresis for metallothioneins analysis in eel liver

被引：10

作者：

Alvarez-Llamas, G ^{[1
]}

Rodríguez-Cea, A ^{[1
]}

de la Campa, MRF ^{[1
]}

Sanz-Medel, A ^{[1
]}

机构：

[1] Univ Oviedo, Dept Physiol & Analyt Chem, E-33006 Oviedo, Spain

来源：

ANALYTICA CHIMICA ACTA | 2003年 / 486卷 / 02期

关键词：

metallothioneins; capillary electrophoresis; sample stacking; cytosol; eel liver;

D O I：

10.1016/S0003-2670(03)00475-6

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A large volume sample stacking procedure (LVSS) is developed here for metallothionein (MT) determinations in rabbit liver by using capillary electrophoresis with UV detection. A 10-time improvement in concentration-based LODs, when compared to the normal stacking mode (CE-UV analysis of samples solved in water), is achieved. The methodology is successfully applied to analysis of MTs in eel liver cytosolic extracts, preceded by an easy cleaning-up pre-treatment in order to eliminate the high salt content. Analysis of cytosol obtained from a group of eels previously exposed to Cd (induced group) resulted in several isoforms of MTs with differences in absorbance signal compared to a control group. (C) 2003 Elsevier Science B.V. All rights reserved.

引用

页码：183 / 190

页数：8

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