Microfluidic genetic analysis with an integrated a-Si:H detector

被引：40

作者：

Kamei, T ^{[1
]}

Toriello, NM ^{[1
]}

Lagally, ET ^{[1
]}

Blazej, RG ^{[1
]}

Scherer, JR ^{[1
]}

Street, RA ^{[1
]}

Mathies, RA ^{[1
]}

机构：

[1] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA

来源：

BIOMEDICAL MICRODEVICES | 2005年 / 7卷 / 02期

关键词：

integrated detector; pathogen detection; PCR; capillary electrophoresis (CE);

D O I：

10.1007/s10544-005-1595-y

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

We have developed an integrated hydrogenated amorphous silicon (a-Si:H) fluorescence detector for microfluidic genetic analysis. It consists of a half-ball lens, a ZnS/YF3 multilayer optical interference filter with a pinhole, and an annular a-Si:H PIN photodiode allowing the laser excitation to pass up through the central aperture in the photodiode and the filter. Microfluidic separations of multiplex PCR products generated from methicillin-resistant/sensitive Staphylococcus aureus (MRSA/MSSA) DNA on microfluidic capillary electrophoresis (CE) devices are successfully detected with the integrated detector. Similarly, multiplex PCR amplicons from the kanamycin resistant and K12 serotype-specific genes of E. coli cells are detected. The direct detection of multiplex PCR amplicons indicates that the fluorescence detector can be successfully coupled with current microfluidic PCR-CE platforms. This work establishes that the integrated a-Si:H detector provides relevant limits of detection for point-of-care genetic and pathogen analysis with microfluidic devices.

引用

页码：147 / 152

页数：6

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[11] Rapid and specific molecular identification of methicillin-resistant Staphylococcus aureus in endotracheal aspirates from mechanically ventilated patients [J].