Trapping intermediates in the crystal: ligand binding to myoglobin

被引：59

作者：

Schlichting, I

Chu, K

机构：

[1] Max Planck Inst Mol Physiol, Dept Phys Biochem, D-44227 Dortmund, Germany

[2] Univ Vermont, Dept Phys, Burlington, VT 05405 USA

来源：

CURRENT OPINION IN STRUCTURAL BIOLOGY | 2000年 / 10卷 / 06期

关键词：

D O I：

10.1016/S0959-440X(00)00158-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Crystal structures of the reactive short-lived species that occur in chemical or binding reactions can be determined using X-ray crystallography via time-resolved or kinetic trapping approaches. Recently, various kinetic trapping methods have been used to determine the structure of intermediates in ligand binding to myoglobin.

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页码：744 / 752

页数：9

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共 60 条

[1] DYNAMICS OF LIGAND-BINDING TO MYOGLOBIN [J].

AUSTIN, RH ;

BEESON, KW ;

EISENSTEIN, L ;

FRAUENFELDER, H ;

GUNSALUS, IC .

BIOCHEMISTRY, 1975, 14 (24) :5355-5373

[2] A mechanism essential to life [J].

Borman, S .

CHEMICAL & ENGINEERING NEWS, 1999, 77 (49) :31-+

[3] Structural dynamics of ligand diffusion in the protein matrix:: A study on a new myoglobin mutant Y(B10) Q(E7) R(E10) [J].

Brunori, M ;

Cutruzzolà, F ;

Savino, C ;

Travaglini-Allocatelli, C ;

Vallone, B ;

Gibson, QH .

BIOPHYSICAL JOURNAL, 1999, 76 (03) :1259-1269

[4] The role of cavities in protein dynamics:: Crystal structure of a photolytic intermediate of a mutant myoglobin [J].

Brunori, M ;

Vallone, B ;

Cutruzzolà, F ;

Travaglini-Allocatelli, C ;

Berendzen, J ;

Chu, K ;

Sweet, RM ;

Schlichting, I .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (05) :2058-2063

[5] Does picosecond protein dynamics have survival value? [J].

Brunori, M ;

Cutruzzola, F ;

Savino, C ;

Travaglini-Allocatelli, C ;

Vallone, B ;

Gibson, QH .

TRENDS IN BIOCHEMICAL SCIENCES, 1999, 24 (07) :253-255

[6] Structural changes in a cryo-cooled protein crystal owing to radiation damage [J].

Burmeister, WP .

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2000, 56 :328-341

[7] The reaction cycle of isopenicillin N synthase observed by X-ray diffraction [J].

Burzlaff, NI ;

Rutledge, PJ ;

Clifton, IJ ;

Hensgens, CMH ;

Pickford, M ;

Adlington, RM ;

Roach, PL ;

Baldwin, JE .

NATURE, 1999, 401 (6754) :721-724

[8] DYNAMICS OF LIGAND-BINDING TO HEME-PROTEINS [J].

CASE, DA ;

KARPLUS, M .

JOURNAL OF MOLECULAR BIOLOGY, 1979, 132 (03) :343-368

[9] XAS spectroscopy reveals X-ray-induced photoreduction of free and protein-bound B12 cofactors [J].

Champloy, F ;

Gruber, K ;

Jogl, G ;

Kratky, C .

JOURNAL OF SYNCHROTRON RADIATION, 2000, 7 (04) :267-273

[10] IDENTIFICATION AND ASSAY OF SYNCHROTRON RADIATION-INDUCED ALTERATIONS ON METALLOENZYMES AND PROTEINS [J].

CHANCE, B ;

ANGIOLILLO, P ;

YANG, EK ;

POWERS, L .

FEBS LETTERS, 1980, 112 (02) :178-182

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