Application of hydrophobic palladium nanoparticles for the development of electrochemical glucose biosensor

被引:26
作者
Li, Zhongping [1 ]
Wang, Xu [1 ]
Wen, Guangming [2 ]
Shuang, Shaomin [2 ]
Dong, Chuan [1 ]
Paau, Man Chin [3 ]
Choi, Martin M. F. [3 ]
机构
[1] Shanxi Univ, Res Ctr Environm Sci & Engn, Taiyuan 030006, Peoples R China
[2] Shanxi Univ, Sch Chem & Chem Engn, Taiyuan 030006, Peoples R China
[3] Hong Kong Baptist Univ, Dept Chem, Kowloon Tong, Hong Kong, Peoples R China
基金
中国国家自然科学基金;
关键词
Palladium nanoparticles; Amperometry; Glucose; HYBRID SYSTEMS; OXIDASE; ELECTRODE; LAYER;
D O I
10.1016/j.bios.2011.04.057
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
An amperometric glucose biosensor based on an n-alkylamine-stabilized palladium nanoparticles (PdNPs)-glucose oxidase (GOx) modified glassy carbon (GC) electrode has been successfully fabricated. PdNPs were initially synthesized by a biphase mixture of water and toluene method using n-alkylamines (dodecylamine. C-12-NH2 and octadecylamine, C-18-NH2) as stabilizing ligands. The performance of the PdNPs-GOx/GC biosensor was studied by cyclic voltammetry. The optimum working potential for amperometric measurement of glucose in pH 7.0 phosphate buffer solution is -0.02 V (vs. Ag/AgCl). The analytical performance of the biosensor prepared from C-18-PdNPs-GOx is better than that of C-12-PdNPs-GOx. The C-18-PdNPs-GOx/GC biosensor exhibits a fast response time of ca. 3 s, a detection limit of 3.0 mu M (S/N = 3) and a linear range of 3.0 mu M-8.0 mM. The linear dependence of current density with glucose concentration is 70.8 mu A cm(-2) mM(-1). The biosensor shows good stability, repeatability and reproducibility. It has been successfully applied to determine the glucose content in human blood serum samples. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码:4619 / 4623
页数:5
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