PHYTOCLOCK 1 encoding a novel GARP protein essential for the Arabidopsis circadian clock

被引:167
作者
Onai, K
Ishiura, M [1 ]
机构
[1] Nagoya Univ, Ctr Gene Res, Chikusa Ku, Nagoya, Aichi 4648602, Japan
[2] Nagoya Univ, Biooriented Technol Res Adv Inst, Chikusa Ku, Nagoya, Aichi 4648602, Japan
[3] Nagoya Univ, Grad Sch Sci, Div Biol Sci, Chikusa Ku, Nagoya, Aichi 4648602, Japan
[4] Aichi Sci & Technol Fdn, Naka Ku, Nagoya, Aichi 4600002, Japan
关键词
D O I
10.1111/j.1365-2443.2005.00892.x
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Previously, we screened 50 000 seedlings of Arabidopsis thaliana carrying a P-GI::LUC+ bioluminescence reporter gene mutagenized with ethylmethanesulfonate for mutants with phenotypes of extensively altered circadian rhythms, and identified three loci, PHYTOCLOCK 1 (PCL1), PCL2 and PCL3, whose mutations cause arrhythmia. Here we succeeded to clone the PCL1 gene and show that the PCL1 gene encodes a novel DNA binding protein belonging to the GARP protein family and is essential for a functional clock oscillator in A. thaliana. The PCL1 gene satisfies the requirements for the clock oscillator gene: (i) pcl1 null mutations caused arrhythmia in multiple circadian outputs, including expression of potential clock genes TOC1, CCA1 and LHY, and flowering lacked a photoperiodic response; (ii) PCL1 expression showed circadian rhythm in both constant light and constant dark; (iii) over-expression of the PCL1 gene gradually caused arrhythmicity in all the multiple circadian outputs examined; and (iv) the PCL1 gene controlled its own expression via negative feedback. Therefore, the PCL1 gene is the clock oscillator gene essential to the generation of clock oscillation in the higher plant.
引用
收藏
页码:963 / 972
页数:10
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