Determination of trace levels of benzodiazepine in urine using capillary electrochromatography-time of flight mass spectrometry

被引:25
作者
Bias, Maximilien [1 ]
McCord, Bruce R. [1 ]
机构
[1] Florida Int Univ, Dept Chem, Miami, FL 33199 USA
关键词
benzodiazepine; electrochromatography; mass spectrometry; monolith;
D O I
10.1002/elps.200700422
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
This paper details a method for the separation and determination of ten benzodiazepines in urine using CEC-MS(TOF) and an hexyl acrylate-based porous monolith. The TOF mass spectrometer provides an exact mass of protonated benzodiazepines to three decimal places (1-6 ppm). This high selectivity along with the CEC separation, provides an effective method for the identification of benzodiazepines. Linearity is satisfactory for all compounds in the concentration range of 25-500 ng/mL for lorazepam and 12.5-500 ng/mL for the others. The RSDs are between 1.4-2.3% for retention times and 1.1-9.2% for relative areas. Using the monolithic stationary phase, a preconcentration step is achievable and permits an 75-140-fold improvement in sensitivity. This strategy permits the quantification of these drugs down to 1 ng/mL in urine. This method was used for the analysis of benzodiazepines in spiked urine samples.
引用
收藏
页码:2182 / 2192
页数:11
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