Use of the 5-cyano-2,3-ditolyl tetrazolium chloride reduction test to assess respiring marine bacteria and grazing effects by flow cytometry during linear alkylbenzene sulfonate degradation

被引:15
作者
López-Amorós, R
Comas, J
García, MT
Vives-Rego, J
机构
[1] Univ Barcelona, Fac Biol, Dept Microbiol, E-08028 Barcelona, Spain
[2] Univ Barcelona, Serv Cientifico Tecn, E-08028 Barcelona, Spain
[3] CSIC, Cid, Dept Tensioact, ES-08034 Barcelona, Spain
关键词
5-cyano-2,3-ditolyl tetrazolium chloride; SYTO-13; flow cytometry; sodium linear alkylbenzene sulfonate; marine bacteria;
D O I
10.1016/S0168-6496(98)00053-1
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Viable, total and metabolically active bacteria were determined during linear alkylbenzene sulfonate degradation in coastal seawater. Viable bacteria were estimated by plate counts on marine agar media while the total and metabolically active bacteria were determined with the nucleic acid stain SYTO-13 and the tetrazolium salt 5-cyano-2,3-ditolyl tetrazolium chloride, respectively, in double stain procedures analyzed by flow cytometry. The double stain SYTO-13/5-cyano-2,3-ditolyl tetrazolium chloride is a rapid and simple method that discriminates bacterioplankton populations according to nucleic acid content and formazan formation. Linear alkylbenzene sulfonate degradation was monitored by high-performance liquid chromatography analysis. Bacterioplankton degraded linear alkylbenzene sulfonate by growing to communities with a high percentage of viable and metabolically active bacteria. The bacteria produced were rapidly grazed by protozoa; however, the grazing took place mostly on metabolically active cells, which were larger than the rest of the population. (C) 1998 Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:33 / 42
页数:10
相关论文
共 29 条
[1]   Relation between presence absence of a visible nucleoid and metabolic activity in bacterioplankton cells [J].
Choi, JW ;
Sherr, EB ;
Sherr, BF .
LIMNOLOGY AND OCEANOGRAPHY, 1996, 41 (06) :1161-1168
[2]   Flow cytometric determination of bacterial abundance in lake plankton with the green nucleic acid stain SYTO 13 [J].
delGiorgio, P ;
Bird, DF ;
Prairie, YT ;
Planas, D .
LIMNOLOGY AND OCEANOGRAPHY, 1996, 41 (04) :783-789
[3]   Bacterioplankton community structure: Protists control net production and the proportion of active bacteria in a coastal marine community [J].
delGiorgio, PA ;
Gasol, JM ;
Vaque, D ;
Mura, P ;
Agusti, S ;
Duarte, CM .
LIMNOLOGY AND OCEANOGRAPHY, 1996, 41 (06) :1169-1179
[4]  
delGiorgio PA, 1997, MICROBIAL ECOL, V34, P144
[5]   THE TETRAZOLIUM REDUCTION METHOD FOR ASSESSING THE VIABILITY OF INDIVIDUAL BACTERIAL-CELLS IN AQUATIC ENVIRONMENTS - IMPROVEMENTS, PERFORMANCE AND APPLICATIONS [J].
DUFOUR, P ;
COLON, M .
HYDROBIOLOGIA, 1992, 232 (03) :211-218
[6]   Active versus inactive bacteria: Size-dependence in a coastal marine plankton community [J].
Gasol, JM ;
delGiorgio, PA ;
Massana, R ;
Duarte, CM .
MARINE ECOLOGY PROGRESS SERIES, 1995, 128 (1-3) :91-97
[7]   Use of nucleic acid dyes SYTO-13, TOTO-1, and YOYO-1 in the study of Escherichia coli and marine prokaryotic populations by flow cytometry [J].
Guindulain, T ;
Comas, J ;
VivesRego, J .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (11) :4608-4611
[8]  
JIMENEZ L, 1991, APPL ENVIRON MICROB, V57, P1566
[9]   THE POTENTIAL IMPORTANCE OF GRAZING-RESISTANT BACTERIA IN PLANKTONIC SYSTEMS [J].
JURGENS, K ;
GUDE, H .
MARINE ECOLOGY PROGRESS SERIES, 1994, 112 (1-2) :169-188
[10]   THE USE OF 5-CYANO-2,3-DITOLYL TETRAZOLIUM CHLORIDE AND FLOW-CYTOMETRY FOR THE VISUALIZATION OF RESPIRATORY ACTIVITY IN INDIVIDUAL CELLS OF MICROCOCCUS-LUTEUS [J].
KAPRELYANTS, AS ;
KELL, DB .
JOURNAL OF MICROBIOLOGICAL METHODS, 1993, 17 (02) :115-122