A Sequential Strand-Displacement Strategy Enables Efficient Six-Step DNA-Templated Synthesis

被引：57

作者：

He, Yu ^{[1
]}

Liu, David R. ^{[1
]}

机构：

[1] Harvard Univ, Howard Hughes Med Inst, Dept Chem & Chem Biol, Cambridge, MA 02138 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2011年 / 133卷 / 26期

关键词：

ENCODED CHEMICAL LIBRARY; IN-VITRO SELECTION; REACTION-DISCOVERY SYSTEM; SMALL-MOLECULE EVOLUTION; NUCLEIC-ACID ALDEHYDES; ORGANIC-SYNTHESIS; TRYPSIN-INHIBITORS; BINDING-MOLECULES; TRANSLATION; POLYMERIZATION;

D O I：

10.1021/ja201361t

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We developed a sequential strand-displacement strategy for multistep DNA-templated synthesis (DTS) and used it to mediate an efficient six-step DTS that proceeded in 35% overall yield (83% average yield per step). The efficiency of this approach and the fact that the final product remains linked to a DNA sequence that fully encodes its reaction history suggests its utility for the translation of DNA sequences into high-complexity synthetic libraries suitable for in vitro selection.

引用

页码：9972 / 9975

页数：4

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