The expression of sterigmatocystin and penicillin genes in Aspergillus nidulans is controlled by veA, a gene required for sexual development

被引:189
作者
Kato, N [1 ]
Brooks, W [1 ]
Calvo, AM [1 ]
机构
[1] No Illinois Univ, Dept Biol Sci, De Kalb, IL 60115 USA
关键词
D O I
10.1128/EC.2.6.1178-1186.2003
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Secondary metabolism is commonly associated with morphological development in microorganisms, including fungi. We found that veA, a gene previously shown to control the Aspergillus nidulans sexual/asexual developmental ratio in response to light, also controls secondary metabolism. Specifically, veA regulates the expression of genes implicated in the synthesis of the mycotoxin sterigmatocystin and the antibiotic penicillin. veA is necessary for the expression of the transcription factor aflR, which activates the gene cluster that leads to the production of sterigmatocystin. veA is also necessary for penicillin production. Our results indicated that although veA represses the transcription of the isopenicillin synthetase gene ipnA, it is necessary for the expression of acvA, the key gene in the first step of penicillin biosynthesis, encoding the delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase. With respect to the mechanism of veA in directing morphological development, veA has little effect on the expression of the known sexual transcription factors nsdD and steA. However, we found that veA regulates the expression of the asexual transcription factor brlA by modulating the alpha/beta transcript ratio that controls conidiation.
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收藏
页码:1178 / 1186
页数:9
相关论文
共 52 条
[31]   Metabolic pathway gene clusters in filamentous fungi [J].
Keller, NP ;
Hohn, TM .
FUNGAL GENETICS AND BIOLOGY, 1997, 21 (01) :17-29
[32]   delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase is a rate limiting enzyme for penicillin production in Aspergillus nidulans [J].
Kennedy, J ;
Turner, G .
MOLECULAR & GENERAL GENETICS, 1996, 253 (1-2) :189-197
[33]   The veA gene activates sexual development in Aspergillus nidulans [J].
Kim, HS ;
Han, KY ;
Kim, KJ ;
Han, DM ;
Jahng, KY ;
Chae, KS .
FUNGAL GENETICS AND BIOLOGY, 2002, 37 (01) :72-80
[34]   THE TUBB ALPHA-TUBULIN GENE IS ESSENTIAL FOR SEXUAL DEVELOPMENT IN ASPERGILLUS-NIDULANS [J].
KIRK, KE ;
MORRIS, NR .
GENES & DEVELOPMENT, 1991, 5 (11) :2014-2023
[35]  
Lee DW, 2001, CURR GENET, V39, P237
[36]   The challenge of antibiotic resistance [J].
Levy, SB .
SCIENTIFIC AMERICAN, 1998, 278 (03) :46-53
[37]   The penicillin regulator PENR1 of Aspergillus nidulans is a HAP-like transcriptional complex [J].
Litzka, O ;
Papagiannopolous, P ;
Davis, MA ;
Hynes, MJ ;
Brakhage, AA .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1998, 251 (03) :758-767
[38]   The riddle of MAP kinase signaling specificity [J].
Madhani, HD ;
Fink, GR .
TRENDS IN GENETICS, 1998, 14 (04) :151-155
[39]   ISOLATION AND TRANSCRIPTIONAL CHARACTERIZATION OF A MORPHOLOGICAL MODIFIER - THE ASPERGILLUS-NIDULANS STUNTED (STUA) GENE [J].
MILLER, KY ;
TOENNIS, TM ;
ADAMS, TH ;
MILLER, BL .
MOLECULAR AND GENERAL GENETICS, 1991, 227 (02) :285-292
[40]  
Papagiannopoulos P, 1996, MOL GEN GENET, V251, P412, DOI 10.1007/s004380050184