Quantum-dot-based electrochemical immunoassay for high-throughput screening of the prostate-specific antigen

被引：102

作者：

Wang, Jun ^{[1
]}

Liu, Guodong ^{[1
]}

Wu, Hong ^{[1
]}

Lin, Yuehe ^{[1
]}

机构：

[1] Pacific NW Natl Lab, Richland, WA 99354 USA

来源：

SMALL | 2008年 / 4卷 / 01期

关键词：

antigens; electrochemical methods; high-throughput screening; immunoassays; quantum dots;

D O I：

10.1002/smll.200700459

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

An electrochemical high-throughput sensing platform for simple, sensitive detection of PSA based on QD labels was investigated. The primary anti-PSA was immobilized on the well of a microplate through electrostatic interaction between the antibody and the polymer coated on the surface of the wall. The secondary PSA antibody was linked to the QD labels by using a bifunctional crosslinker. The prepared conjugates were characterized by UV spectroscopy. The anti-PSA-QD conjugates were further characterized with transmission electron microscopy (TEM). A small signal was also observed in the serum with no PSA. The effects of the concentration of primary anti-PSA on electrochemical response of PSA was also studied. It was observed that the voltammetric response of PSA increases with the increase of anti-PSA concentration.

引用

页码：82 / 86

页数：5

共 34 条

[1] Development and validation of a quantitative ELISA for the measurement of PSA concentration [J].