Potential role of nuclear PD-L1 expression in cell-surface vimentin positive circulating tumor cells as a prognostic marker in cancer patients

被引：184

作者：

Satelli, Arun ^{[1
]}

Batth, Izhar Singh ^{[1
]}

Brownlee, Zachary ^{[1
]}

Rojas, Christina ^{[1
]}

Meng, Qing H. ^{[2
]}

Kopetz, Scott ^{[3
,4
]}

Li, Shulin ^{[1
,5
]}

机构：

[1] Univ Texas MD Anderson Canc Ctr, Dept Pediat, Houston, TX 77030 USA

[2] Univ Texas MD Anderson Canc Ctr, Dept Lab Med, Houston, TX 77030 USA

[3] Univ Texas MD Anderson Canc Ctr, Dept Surg Oncol, Houston, TX 77030 USA

[4] Univ Texas MD Anderson Canc Ctr, Dept Mol & Cellular Oncol, Houston, TX 77030 USA

[5] Univ Texas Grad Sch Biomed Sci, Houston, TX USA

来源：

SCIENTIFIC REPORTS | 2016年 / 6卷

关键词：

BREAST-CANCER; PDL1; EXPRESSION; CELLSEARCH(R); ENUMERATION; FREQUENT; B7-H1; GUIDE; EMT;

D O I：

10.1038/srep28910

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Although circulating tumor cells (CTCs) have potential as diagnostic biomarkers for cancer, determining their prognostic role in cancer patients undergoing treatment is a challenge. We evaluated the prognostic value of programmed death-ligand 1 (PD(L1) expression in CTCs in colorectal and prostate cancer patients undergoing treatment. Peripheral blood samples were collected from 62 metastatic colorectal cancer patients and 30 metastatic prostate cancer patients. CTCs were isolated from the samples using magnetic separation with the cell(surface vimentin(CSV)(specific 84(1 monoclonal antibody that detects epithelial(mesenchymal transitioned (EMT) CTCs. CTCs were enumerated and analyzed for PD(L1 expression using confocal microscopy. PD(L1 expression was detectable in CTCs and was localized in the membrane and/or cytoplasm and nucleus. CTC detection alone was not associated with poor progression(free or overall survival in colorectal cancer or prostate cancer patients, but nuclear PD(L1 (nPD(L1) expression in these patients was significantly associated with short survival durations. These results demonstrated that nPD(L1 has potential as a clinically relevant prognostic biomarker for colorectal and prostate cancer. Our data thus suggested that use of CTC(based models of cancer for risk assessment can improve the standard cancer staging criteria and supported the incorporation of nPD(L1 expression detection in CTCs detection in such models.

引用

页数：7

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