Purine synthesis and increased Agrobacterium tumefaciens transformation of yeast and plants

被引：24

作者：

Roberts, RL ^{[1
]}

Metz, M ^{[1
]}

Monks, DE ^{[1
]}

Mullaney, ML ^{[1
]}

Hall, T ^{[1
]}

Nester, EW ^{[1
]}

机构：

[1] Univ Washington, Dept Microbiol, Seattle, WA 98195 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2003年 / 100卷 / 11期

关键词：

D O I：

10.1073/pnas.1132022100

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The bacterium Agrobacterium, tumefaciens transforms eukaryotic hosts by transferring DNA to the recipient cell where it is integrated and expressed. Bacterial factors involved in this interkingdom gene transfer have been described, but less is known about host-cell factors. Using the yeast Saccharomyces cerevisiae as a model host, we devised a genetic screen to identify yeast mutants with altered transformation sensitivities. Twenty-four adenine auxotrophs were identified that exhibited supersensitivity to A. tumefaciens-mediated transformation when deprived of adenine. We extended these results to plants by showing that purine synthesis inhibitors cause supersensitivity to A. tumiefaciens transformation in three plant species. The magnitude of this effect is large and does not depend on prior genetic manipulations of host cells. These data indicate the utility of yeast as a model for the transformation process and identify purine biosynthesis as a key determinant of transformation efficiency. These findings should increase the utility of A. tumefaciens in genetic engineering.

引用

页码：6634 / 6639

页数：6

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