Effect of acyl donor chain length and sugar/acyl donor molar ratio on enzymatic synthesis of fatty acid fructose esters

被引:58
作者
Soultani, S [1 ]
Engasser, JM [1 ]
Ghoul, M [1 ]
机构
[1] INPL, ENSAIA, Grp Lipoprocedes, F-54505 Vandoeuvre Nancy, France
关键词
sugar esters; Candida antarctica lipase; direct esterification; sugar/acyl donor molar ratio; fatty acid chain length; experimental design;
D O I
10.1016/S1381-1177(00)00162-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Lipase-catalyzed synthesis of fatty acid sugar esters through direct esterification was performed in 2-methyl 2-butanol as solvent. Fructose and saturated fatty acids were used as substrates and the reaction was catalyzed by immobilized Candida antarctica lipase. The effect of the initial fructose/acyl donor molar ratio and the carbon-chain length of the acyl donor as well as their reciprocal interactions on the reaction performance were investigated. For this purpose, an experimental design taking into account variations of the molar ratio (from 1:1 to 1:5) and the carbon-chain length of the fatty acid (from C8 to C18) was employed. Statistical analysis of the data indicated that the two factors as well as their interactions had significant effects on the sugar esters synthesis. The obtained results showed that whatever the molar ratio used, the highest concentration (73 g l(-1)), fructose and fatty acid conversion yields (100% and 80%, respectively) and initial reaction rate (40 g l(-1) h(-1)) were reached when using the Cls fatty acid as acyl donor. Low molar ratios gave the best fatty acid conversion yields and initial reaction rates, whereas the best total sugar ester concentrations and fructose conversion yields were obtained for high molar ratios. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:725 / 731
页数:7
相关论文
共 19 条
[1]   Quantitative enzymatic production of 1,6-diacyl fructofuranoses [J].
Arcos, JA ;
Bernabe, M ;
Otero, C .
ENZYME AND MICROBIAL TECHNOLOGY, 1998, 22 (01) :27-35
[2]   FACILE SYNTHESIS OF FATTY-ACID ESTERS IN HIGH YIELDS [J].
BLOOMER, S ;
ADLERCREUTZ, P ;
MATTIASSON, B .
ENZYME AND MICROBIAL TECHNOLOGY, 1992, 14 (07) :546-552
[3]   Lipase-catalyzed solid phase synthesis of sugar fatty acid esters [J].
Cao, LQ ;
Fischer, A ;
Bornscheuer, UT ;
Schmid, RD .
BIOCATALYSIS AND BIOTRANSFORMATION, 1997, 14 (04) :269-283
[4]   EFFECT OF WATER ACTIVITY ON ENZYMATIC-SYNTHESIS OF ALKYLGLYCOSIDES [J].
CHAHID, Z ;
MONTET, D ;
PINA, M ;
GRAILLE, J .
BIOTECHNOLOGY LETTERS, 1992, 14 (04) :281-284
[5]   PRODUCTION OF BIOSURFACTANTS FROM SUGAR ALCOHOLS AND VEGETABLE-OILS CATALYZED BY LIPASES IN A NONAQUEOUS MEDIUM [J].
CHOPINEAU, J ;
MCCAFFERTY, FD ;
THERISOD, M ;
KLIBANOV, AM .
BIOTECHNOLOGY AND BIOENGINEERING, 1988, 31 (03) :208-214
[6]   COMPARISON OF DIRECT ESTERIFICATION AND TRANSESTERIFICATION OF FRUCTOSE BY CANDIDA-ANTARTICA LIPASE [J].
COULON, D ;
GIRARDIN, M ;
ROVEL, B ;
GHOUL, M .
BIOTECHNOLOGY LETTERS, 1995, 17 (02) :183-186
[7]   Effect of different biochemical parameters on the enzymatic synthesis of fructose oleate [J].
Coulon, D ;
Ismail, A ;
Girardin, M ;
Rovel, B ;
Ghoul, M .
JOURNAL OF BIOTECHNOLOGY, 1996, 51 (02) :115-121
[8]   Effect of the thermodynamic water activity and the reaction medium hydrophobicity on the enzymatic synthesis of ascorbyl palmitate [J].
Humeau, C ;
Girardin, M ;
Rovel, B ;
Miclo, A .
JOURNAL OF BIOTECHNOLOGY, 1998, 63 (01) :1-8
[9]   Enzymatic synthesis of butylglycosides by glycosidases [J].
Ismail, A ;
Ghoul, M .
BIOTECHNOLOGY LETTERS, 1996, 18 (10) :1199-1204
[10]  
JUNG S, 1998, J SURFACTANTS DETERG, V1, P53