Structure of an RNA internal loop consisting of tandem C-A+ base pairs

被引：62

作者：

Jang, SB ^{[1
]}

Hung, LW ^{[1
]}

Chi, YI ^{[1
]}

Holbrook, EL ^{[1
]}

Carter, RJ ^{[1
]}

Holbrook, SR ^{[1
]}

机构：

[1] Univ Calif Berkeley, Phys Biosci Div, Dept Biol Struct, Lawrence Berkeley Lab, Berkeley, CA 94720 USA

来源：

BIOCHEMISTRY | 1998年 / 37卷 / 34期

关键词：

D O I：

10.1021/bi980758j

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The crystal structure of the RNA octamer 5'-CGC(CA)GCG-3' has been determined from X-ray diffraction data to 2.3 Angstrom resolution. In the crystal, this oligomer forms a self-complementary double helix in the asymmetric unit. Tandem non-Watson-Crick C-A and A-C base pairs comprise an internal loop in the middle of the duplex, which is incorporated with little distortion of the A-form double helix. From the geometry of the C-A base pairs, it is inferred that the adenosine imino group is protonated and donates a hydrogen bond to the carbonyl group of the cytosine. The wobble geometry of the C-A(+) base pairs is very similar to that of the common U-G non-Watson-Crick pair.

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页码：11726 / 11731

页数：6

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