Design and characterization of a homodimeric antiparallel coiled coil

被引：66

作者：

Gurnon, DG ^{[1
]}

Whitaker, JA ^{[1
]}

Oakley, MG ^{[1
]}

机构：

[1] Indiana Univ, Dept Chem, Bloomington, IN 47405 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2003年 / 125卷 / 25期

关键词：

D O I：

10.1021/ja0357590

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report the first successful design of a self-associating antiparallel coiled coil, APH. The simultaneous application of Coulombic and hydrophobic components results in a decided preference for the antiparallel alignment as judged by HPLC, sedimentation equilibrium, and chemical denaturation data. The designed peptide is of comparable stability to naturally occurring leucine zipper peptides and can be expressed in bacteria. These properties of APH suggest potential in vivo protein fusion and biomaterials applications. Copyright © 2003 American Chemical Society.

引用

页码：7518 / 7519

页数：2

共 32 条

[1] A heterodimerizing leucine zipper coiled coil system for examining the specificity of a position interactions: Amino acids I, V, L, N, A, and K
Acharya, A
Ruvinov, SB
Gal, J
Moll, JR
Vinson, C
[J]. BIOCHEMISTRY, 2002, 41 (48) : 14122 - 14131
[2] NATIVE-LIKE AND STRUCTURALLY CHARACTERIZED DESIGNED ALPHA-HELICAL BUNDLES
BETZ, SF
BRYSON, JW
DEGRADO, WF
[J]. CURRENT OPINION IN STRUCTURAL BIOLOGY, 1995, 5 (04) : 457 - 463
[3] Coiled coils: a highly versatile protein folding motif
Burkhard, P
Stetefeld, J
Strelkov, SV
[J]. TRENDS IN CELL BIOLOGY, 2001, 11 (02) : 82 - 88
[4] Complementation of buried lysine and surface polar residues in a designed heterodimeric coiled coil
Campbell, KM
Lumb, KJ
[J]. BIOCHEMISTRY, 2002, 41 (22) : 7169 - 7175
[5] Use of a heterodimeric coiled-coil system for biosensor application and affinity purification
Chao, HM
Bautista, DL
Litowski, J
Irvin, RT
Hodges, RS
[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 1998, 715 (01): : 307 - 329
[6] A 2ND CLASS OF SYNTHETASE STRUCTURE REVEALED BY X-RAY-ANALYSIS OF ESCHERICHIA-COLI SERYL-TRANSFER RNA-SYNTHETASE AT 2.5-A
CUSACK, S
BERTHETCOLOMINAS, C
HARTLEIN, M
NASSAR, N
LEBERMAN, R
[J]. NATURE, 1990, 347 (6290) : 249 - 255
[7] De novo design and structural characterization of proteins and metalloproteins
DeGrado, WF
Summa, CM
Pavone, V
Nastri, F
Lombardi, A
[J]. ANNUAL REVIEW OF BIOCHEMISTRY, 1999, 68 : 779 - 819
[8] Mechanisms of viral membrane fusion and its inhibition
Eckert, DM
Kim, PS
[J]. ANNUAL REVIEW OF BIOCHEMISTRY, 2001, 70 : 777 - 810
[9] THE ALACOIL - A VERY TIGHT, ANTIPARALLEL COILED-COIL OF HELICES
GERNERT, KM
SURLES, MC
LABEAN, TH
RICHARDSON, JS
RICHARDSON, DC
[J]. PROTEIN SCIENCE, 1995, 4 (11) : 2252 - 2260
[10] A SWITCH BETWEEN 2-STRANDED, 3-STRANDED AND 4-STRANDED COILED COILS IN GCN4 LEUCINE-ZIPPER MUTANTS
HARBURY, PB
ZHANG, T
KIM, PS
ALBER, T
[J]. SCIENCE, 1993, 262 (5138) : 1401 - 1407

← 1 2 3 4 →