The thiocarbamate-inducible Rhodococcus enzyme ThcF as a member of the family of α/β hydrolases with haloperoxidative side activity

被引：8

作者：

De Mot, R ^{[1
]}

De Schrijver, A ^{[1
]}

Schoofs, G ^{[1
]}

Parret, AHA ^{[1
]}

机构：

[1] Catholic Univ Louvain, Dept Appl Platn Sci, Ctr Microbial & Plant Genet, B-3001 Heverlee, Belgium

来源：

FEMS MICROBIOLOGY LETTERS | 2003年 / 224卷 / 02期

关键词：

alpha/beta hydrolase; esterase; non-heme haloperoxidase; thiocarbamate herbicide; LAL regulator family; HASH family;

D O I：

10.1016/S0378-1097(03)00452-X

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Purified thiocarbamate-inducible ThcF of Rhodococcus erythropolis N186/21, overexpressed in Escherichia coli, displayed several characteristics of the HASH family of enzymes that groups prokaryotic proteins of the alp hydrolase superfamily possessing serine-dependent hydrolase and/or haloperoxidase activity. Kinetic analysis of bromination and ester hydrolysis revealed a low affinity of ThcF for model substrates. Sulfoxidation of thiocarbamates was demonstrated but probably represents a side activity due to peroxoacid generation by the enzyme. The thcF-linked thcG gene, encoding a LAL-type regulator, triggers expression of thcF in Rhodococcus. The tandem gene organization thcG-thcF is conserved in the thiocarbamate-degrading strain Rhodococcus sp. 1330. It is proposed that HASH enzymes may be involved in the metabolism of plant-derived compounds. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

收藏

页码：197 / 203

页数：7

相关论文

共 39 条

[11] Structural investigation of the cofactor-free chloroperoxidases [J].

Hofmann, B ;

Tölzer, S ;

Pelletier, I ;

Altenbuchner, J ;

van Pée, KH ;

Hecht, HJ .

JOURNAL OF MOLECULAR BIOLOGY, 1998, 279 (04) :889-900

[12] Role of Acinetobacter calcoaceticus 3,4-dihydrocoumarin hydrolase in oxidative stress defence against peroxoacids [J].

Honda, K ;

Kataoka, M ;

Sakuradani, E ;

Shimizu, S .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 2003, 270 (03) :486-494

[13] Enzymatic preparation of D-β-acetylthioisobutyric acid and cetraxate hydrochloride using a stereo- and/or regioselective hydrolase, 3,4-dihydrocoumarin hydrolase from Acinetobacter calcoaceticus [J].

Honda, K ;

Kataoka, M ;

Shimizu, S .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2002, 60 (03) :288-292

[14] Cloning and biochemical characterization of Co2+-activated bromoperoxidase-esterase (perhydrolase) from Pseudomonas putida IF-3 strain [J].

Itoh, N ;

Kawanami, T ;

Liu, JQ ;

Dairi, T ;

Miyakoshi, M ;

Nitta, C ;

Kimoto, Y .

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 2001, 1545 (1-2) :53-66

[15] Antifungal and peroxidative activities of nonheme chloroperoxidase in relation to transgenic plant protection [J].

Jacks, TJ ;

De Lucca, AJ ;

Rajasekaran, K ;

Stromberg, K ;

van Pée, KH .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2000, 48 (10) :4561-4564

[16] Evaluation of peracid formation as the basis for resistance to infection in plants transformed with haloperoxidase [J].

Jacks, TJ ;

Rajasekaran, K ;

Stromberg, KD ;

De Lucca, AJ ;

van Pée, KH .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2002, 50 (04) :706-709

[17] 3,4-Dihydrocoumarin hydrolase with haloperoxidase activity from Acinetobacter calcoaceticus F46 [J].

Kataoka, M ;

Honda, K ;

Shimizu, S .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 2000, 267 (01) :3-10

[18] Reaction mechanism of the Co2+-activated multifunctional bromoperoxidase-esterase from Pseudomonas putida IF-3 [J].

Kawanami, T ;

Miyakoshi, M ;

Dairi, T ;

Itoh, N .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2002, 398 (01) :94-100

[19] The non-haem chloroperoxidase from Pseudomonas fluorescens and its relationship to pyrrolnitrin biosynthesis [J].

Kirner, S ;

Krauss, S ;

Sury, G ;

Lam, ST ;

Ligon, JM ;

vanPee, KH .

MICROBIOLOGY-UK, 1996, 142 :2129-2135

[20] Screening for novel enzymes for biocatalytic processes: accessing the metagenome as a resource of novel functional sequence space [J].

Lorenz, P ;

Liebeton, K ;

Niehaus, F ;

Eck, J .

CURRENT OPINION IN BIOTECHNOLOGY, 2002, 13 (06) :572-577

← 1 2 3 4 →