Synthesis of a novel histidine analogue and its efficient incorporation into a protein in vivo

被引：30

作者：

Ikeda, Y

Kawahara, S

Taki, M

Kuno, A

Hasegawa, T

Taira, K

机构：

[1] Univ Tokyo, Sch Engn, Dept Chem & Biotechnol, Tokyo 1138656, Japan

[2] Natl Inst Adv Ind Sci & Technol, Gene Funct Res Ctr, Tukuba Sci City 3058562, Japan

[3] Yamagata Univ, Fac Sci, Dept Mat & Biol Chem, Yamagata 9908560, Japan

来源：

PROTEIN ENGINEERING | 2003年 / 16卷 / 09期

关键词：

amino acid analogue; histidine; incorporation; translation; 1,2,3-triazole;

D O I：

10.1093/protein/gzg084

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Proteins containing unnatural amino acids have immense potential in biotechnology and medicine. We prepared several histidine analogues including a novel histidine analogue, beta-(1,2,3-triazol-4-yl)-dl-alanine. These histidine analogues were assayed for translational activity in histidine-auxotrophic Escherichia coli strain UTH780. We observed that several histidine analogues, including our novel histidine analogue, were efficiently incorporated into the protein in vivo; however, other analogues were rejected. These results suggest that the hydrogen atom at a specific position seriously affects incorporation.

引用

页码：699 / 706

页数：8

共 25 条

[1] The synthesis of pyrimidin-4-yl substituted α-amino acids.: A versatile approach from alkynyl ketones [J].