Examination of In vitro and In vivo biocompatibility of alginate-hyaluronic acid microbeads As a promising method in cell delivery for kidney regeneration

被引：33

作者：

Amirian, Jhaleh ^{[1
]}

Van, Tran Thi Tuong ^{[2
]}

Bae, Sang-Ho ^{[1
,3
]}

Jung, Hae-Il ^{[1
,3
]}

Choi, Hwan-Jun ^{[1
,4
]}

Cho, Hyun-Deuk ^{[5
]}

Lee, Byong-Taek ^{[1
,2
]}

机构：

[1] Soonchunhyang Univ, Inst Tissue Regenerat, 366-1 Ssangyong Dong, Cheonan 330090, Chungcheongnam, South Korea

[2] Soonchunhyang Univ, Dept Regenerat Med, Coll Med, 366-1 Ssangyong Dong, Cheonan 330090, Chungcheongnam, South Korea

[3] Soonchunhyang Univ Hosp, Dept Surg, Coll Med, Cheonan, South Korea

[4] Soonchunhyang Univ Hosp, Dept Plast & Reconstruct Surg, Coll Med, Cheonan, South Korea

[5] Soonchunhyang Univ, Cheonan Hosp, Dept Pathol, Coll Med, Cheonan, South Korea

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2017年 / 105卷

基金：

新加坡国家研究基金会;

关键词：

ALG-HA microbeads; RBMSCs; Encapsulation; In vivo biocompatibility; Kidney; MESENCHYMAL STEM-CELLS; DRUG-DELIVERY; BIOMEDICAL APPLICATIONS; GROWTH-FACTOR; BONE-MARROW; HYDROGEL; TISSUE; INJURY; RAT; RELEASE;

D O I：

10.1016/j.ijbiomac.2017.07.019

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

In this study, alginate (ALG) and alginate-hyaluronic acid (ALG-HA) injectable microbeads, with the purpose of delivering stem cells for tissue engineering, were prepared by a spraying method into a CaCl2 solution that shows high porosity for the exchange of nutrition and waste. In addition, the size distribution and surface morphology was investigated using optical and scanning electron microscopy, respectively. The chemical structural properties of the ALG-HA microbeads were examined by Fourier transform infrared spectroscopy. The biocompatibility of ALG and ALG-HA microbeads was examined in vitro. Rat bone marrow stem cells were encapsulated in microbeads to investigate cell release, cell viability, proliferation, and secretion of growth factors such as VEGF and PDGF. Growth factors were released for the 21 day experimental period. Cells were found to be released from the microbeads after 7 days. Furthermore, the in vivo biocompatibility of ALG-HA microbeads was examined using microbeads without cell encapsulation in the kidney capsule, in order to assess the foreign body reaction and inflammatory response, for 14days. The desired in vivo response to ALG-HA microbeads hydrogel makes it an exquisite candidate for subcapsular cell and drug delivery to kidney tissue. (C) 2017 Published by Elsevier B.V.

引用

页码：143 / 153

页数：11

共 58 条

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In Vivo Study of a Blended Hydrogel Composed of Pluronic F-127-Alginate-Hyaluronic Acid for its Cell Injection Application [J].