Cleavage and isomerization of UpU promoted by dinuclear metal ion complexes

被引：58

作者：

Linjalahti, Heidi ^{[1
]}

Feng, Guoqiang ^{[2
]}

Mareque-Rivas, Juan C. ^{[3
]}

Mikkola, Satu ^{[1
]}

Williams, Nicholas H. ^{[2
]}

机构：

[1] Univ Turku, Dept Chem, FI-20014 Turku, Finland

[2] Univ Sheffield, Dept Chem, Ctr Chem Biol, Sheffield S3 7HF, S Yorkshire, England

[3] Univ Edinburgh, Sch Chem, Edinburgh EH9 3JJ, Midlothian, Scotland

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2008年 / 130卷 / 13期

基金：

英国工程与自然科学研究理事会;

关键词：

D O I：

10.1021/ja711347w

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The catalysis of phosphoryl transfer by metal ions has been intensively studied in both biological and artificial systems, but the status of the transient pentacoordinate phosphoryl species (as transition state or intermediate) is the subject of considerable debate. We report that dinuclear metal ion complexes that incorporate second sphere hydrogen bond donors not only promote the cleavage of RNA fragments just as efficiently as the activated analogue HPNPP but also provide the first examples of metal ion catalyzed phosphate diester isomerization close to neutral pH. This observation implies that the reaction catalyzed by these complexes involves the formation of a phosphorane intermediate that is sufficiently long-lived to pseudorotate.

引用

收藏

页码：4232 / +

页数：3

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