BacA of Sinorhizobium meliloti plays an essential role in the establishment of nitrogen-fixing symbioses with Medicago plants, where it is involved in peptide import and in the addition of very-long-chain fatty acids (VLCFA) to lipid A of lipopolysaccharide (LPS). We investigated the role of BacA in Rhizobium species strain NGR234 by mutating the bacA gene. In the NGR234 bacA mutant, peptide import was impaired, but no effect on VLCFA addition was observed. More importantly, the symbiotic ability of the mutant was comparable to that of the wild type for a variety of legume species. Concurrently, an acpXL mutant of NGR234 was created and assayed. In rhizobia, AcpXL is a dedicated acyl carrier protein necessary for the addition of VLCFA to lipid A. LPS extracted from the NGR234 mutant lacked VLCFA, and this mutant was severely impaired in the ability to form functional nodules with the majority of legumes tested. Our work demonstrates the importance of VLCFA in the NGR234-legume symbiosis and also shows that the necessity of BacA for bacteroid differentiation is restricted to specific legume-Rhizobium interactions.
机构:
CNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, FranceCNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France
Van de Velde, Willem
;
Zehirov, Grigor
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Kagoshima Univ, Grad Sch Sci & Engn, Kagoshima 8900065, JapanCNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France
Zehirov, Grigor
;
Szatmari, Agnes
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CNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France
Hungarian Acad Sci, Inst Plant Protect, H-1022 Budapest, HungaryCNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France
Szatmari, Agnes
;
Debreczeny, Monika
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Bay Zoltan Fdn Appl Res, Inst Plant Genom Human Biotechnol & Bioenergy, H-6726 Szeged, HungaryCNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France
Debreczeny, Monika
;
Ishihara, Hironobu
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Kagoshima Univ, Grad Sch Sci & Engn, Kagoshima 8900065, JapanCNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France
Ishihara, Hironobu
;
Kevei, Zoltan
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Bay Zoltan Fdn Appl Res, Inst Plant Genom Human Biotechnol & Bioenergy, H-6726 Szeged, HungaryCNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France
Kevei, Zoltan
;
Farkas, Attila
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h-index: 0
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Bay Zoltan Fdn Appl Res, Inst Plant Genom Human Biotechnol & Bioenergy, H-6726 Szeged, HungaryCNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France
Farkas, Attila
;
Mikulass, Kata
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Bay Zoltan Fdn Appl Res, Inst Plant Genom Human Biotechnol & Bioenergy, H-6726 Szeged, HungaryCNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France
Mikulass, Kata
;
Nagy, Andrea
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Bay Zoltan Fdn Appl Res, Inst Plant Genom Human Biotechnol & Bioenergy, H-6726 Szeged, HungaryCNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France
Nagy, Andrea
;
Tiricz, Hilda
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Bay Zoltan Fdn Appl Res, Inst Plant Genom Human Biotechnol & Bioenergy, H-6726 Szeged, HungaryCNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France
Tiricz, Hilda
;
Satiat-Jeunemaitre, Beatrice
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CNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, FranceCNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France
Satiat-Jeunemaitre, Beatrice
;
Alunni, Benoit
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CNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, FranceCNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France
Alunni, Benoit
;
Bourge, Mickael
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CNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, FranceCNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France
Bourge, Mickael
;
Kucho, Ken-ichi
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Kagoshima Univ, Grad Sch Sci & Engn, Kagoshima 8900065, JapanCNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France
Kucho, Ken-ichi
;
Abe, Mikiko
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Kagoshima Univ, Grad Sch Sci & Engn, Kagoshima 8900065, JapanCNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France
Abe, Mikiko
;
Kereszt, Attila
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Bay Zoltan Fdn Appl Res, Inst Plant Genom Human Biotechnol & Bioenergy, H-6726 Szeged, HungaryCNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France
Kereszt, Attila
;
Maroti, Gergely
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Bay Zoltan Fdn Appl Res, Inst Plant Genom Human Biotechnol & Bioenergy, H-6726 Szeged, HungaryCNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France
Maroti, Gergely
;
Uchiumi, Toshiki
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h-index: 0
机构:
Kagoshima Univ, Grad Sch Sci & Engn, Kagoshima 8900065, JapanCNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France
Uchiumi, Toshiki
;
Kondorosi, Eva
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h-index: 0
机构:
CNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France
Bay Zoltan Fdn Appl Res, Inst Plant Genom Human Biotechnol & Bioenergy, H-6726 Szeged, HungaryCNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France
Kondorosi, Eva
;
Mergaert, Peter
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h-index: 0
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CNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, FranceCNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France
机构:
CNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, FranceCNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France
Van de Velde, Willem
;
Zehirov, Grigor
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h-index: 0
机构:
Kagoshima Univ, Grad Sch Sci & Engn, Kagoshima 8900065, JapanCNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France
Zehirov, Grigor
;
Szatmari, Agnes
论文数: 0引用数: 0
h-index: 0
机构:
CNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France
Hungarian Acad Sci, Inst Plant Protect, H-1022 Budapest, HungaryCNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France
Szatmari, Agnes
;
Debreczeny, Monika
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h-index: 0
机构:
Bay Zoltan Fdn Appl Res, Inst Plant Genom Human Biotechnol & Bioenergy, H-6726 Szeged, HungaryCNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France
Debreczeny, Monika
;
Ishihara, Hironobu
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h-index: 0
机构:
Kagoshima Univ, Grad Sch Sci & Engn, Kagoshima 8900065, JapanCNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France
Ishihara, Hironobu
;
Kevei, Zoltan
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h-index: 0
机构:
Bay Zoltan Fdn Appl Res, Inst Plant Genom Human Biotechnol & Bioenergy, H-6726 Szeged, HungaryCNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France
Kevei, Zoltan
;
Farkas, Attila
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h-index: 0
机构:
Bay Zoltan Fdn Appl Res, Inst Plant Genom Human Biotechnol & Bioenergy, H-6726 Szeged, HungaryCNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France
Farkas, Attila
;
Mikulass, Kata
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Bay Zoltan Fdn Appl Res, Inst Plant Genom Human Biotechnol & Bioenergy, H-6726 Szeged, HungaryCNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France
Mikulass, Kata
;
Nagy, Andrea
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h-index: 0
机构:
Bay Zoltan Fdn Appl Res, Inst Plant Genom Human Biotechnol & Bioenergy, H-6726 Szeged, HungaryCNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France
Nagy, Andrea
;
Tiricz, Hilda
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h-index: 0
机构:
Bay Zoltan Fdn Appl Res, Inst Plant Genom Human Biotechnol & Bioenergy, H-6726 Szeged, HungaryCNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France
Tiricz, Hilda
;
Satiat-Jeunemaitre, Beatrice
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h-index: 0
机构:
CNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, FranceCNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France
Satiat-Jeunemaitre, Beatrice
;
Alunni, Benoit
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h-index: 0
机构:
CNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, FranceCNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France
Alunni, Benoit
;
Bourge, Mickael
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h-index: 0
机构:
CNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, FranceCNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France
Bourge, Mickael
;
Kucho, Ken-ichi
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h-index: 0
机构:
Kagoshima Univ, Grad Sch Sci & Engn, Kagoshima 8900065, JapanCNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France
Kucho, Ken-ichi
;
Abe, Mikiko
论文数: 0引用数: 0
h-index: 0
机构:
Kagoshima Univ, Grad Sch Sci & Engn, Kagoshima 8900065, JapanCNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France
Abe, Mikiko
;
Kereszt, Attila
论文数: 0引用数: 0
h-index: 0
机构:
Bay Zoltan Fdn Appl Res, Inst Plant Genom Human Biotechnol & Bioenergy, H-6726 Szeged, HungaryCNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France
Kereszt, Attila
;
Maroti, Gergely
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h-index: 0
机构:
Bay Zoltan Fdn Appl Res, Inst Plant Genom Human Biotechnol & Bioenergy, H-6726 Szeged, HungaryCNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France
Maroti, Gergely
;
Uchiumi, Toshiki
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h-index: 0
机构:
Kagoshima Univ, Grad Sch Sci & Engn, Kagoshima 8900065, JapanCNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France
Uchiumi, Toshiki
;
Kondorosi, Eva
论文数: 0引用数: 0
h-index: 0
机构:
CNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France
Bay Zoltan Fdn Appl Res, Inst Plant Genom Human Biotechnol & Bioenergy, H-6726 Szeged, HungaryCNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France
Kondorosi, Eva
;
Mergaert, Peter
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h-index: 0
机构:
CNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, FranceCNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France