Genome-wide transcriptional responses of Escherichia coli k-12 to continuous osmotic and heat stresses

被引:144
作者
Gunasekera, Thusitha S. [1 ]
Csonka, Laszlo N. [2 ]
Paliy, Oleg [1 ]
机构
[1] Wright State Univ, Dept Biochem & Mol Biol, Dayton, OH 45435 USA
[2] Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA
关键词
D O I
10.1128/JB.01990-07
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Osmotic stress is known to increase the thermotolerance and oxidative-stress resistance of bacteria by a mechanism that is not adequately understood. We probed the cross-regulation of continuous osmotic and heat stress responses by characterizing the effects of external osmolarity (0.3 M versus 0.0 M NaCl) and temperature (43 degrees C versus 30 degrees C) on the transcriptome of Escherichia coli K-12. Our most important discovery was that a number of genes in the SoxRS and OxyR oxidative-stress regulons were up-regulated by high osmolarity, high temperature, or a combination of both stresses. This result can explain the previously noted cross-protection of osmotic stress against oxidative and heat stresses. Most of the genes shown in previous studies to be induced during the early phase of adaptation to hyperosmotic shock were found to be also overexpressed under continuous osmotic stress. However, there was a poorer overlap between the heat shock genes that are induced transiently after high temperature shifts and the genes that we found to be chronically up-regulated at 43 degrees C. Supplementation of the high-osmolarity medium with the osmoprotectant glycine betaine, which reduces the cytoplasmic K+ pool, did not lead to a universal reduction in the expression of osmotically induced genes. This finding does not support the hypothesis that K+ is the central osmoregulatory signal in Enterobacteriaceae.
引用
收藏
页码:3712 / 3720
页数:9
相关论文
共 68 条
[61]   Role of putrescine in regulation of the σS subunit of RNA polymerase in Escherichia coli cells on transition to stationary phase [J].
Tkachenko, AG ;
Shumkov, MS .
BIOCHEMISTRY-MOSCOW, 2004, 69 (08) :876-882
[62]   Exogenous glutathione completes the defense against oxidative stress in Haemophilus influenzae [J].
Vergauwen, B ;
Pauwels, F ;
Vaneechoutte, M ;
Van Beeumen, JJ .
JOURNAL OF BACTERIOLOGY, 2003, 185 (05) :1572-1581
[63]   Profiling early osmostress-dependent gene expression in Escherichia coli using DNA microarrays [J].
Weber, A ;
Jung, K .
JOURNAL OF BACTERIOLOGY, 2002, 184 (19) :5502-5507
[64]   Time-dependent proteome alterations under osmotic stress during aerobic and anaerobic growth in Escherichia coli [J].
Weber, Arnim ;
Koegl, Stephanie A. ;
Jung, Kirsten .
JOURNAL OF BACTERIOLOGY, 2006, 188 (20) :7165-7175
[65]  
Wick LM, 2004, ADV BIOCHEM ENG BIOT, V89, P1
[66]   Osmosensing and osmoregulatory compatible solute accumulation by bacteria [J].
Wood, JM ;
Bremer, E ;
Csonka, LN ;
Kraemer, R ;
Poolman, B ;
van der Heide, T ;
Smith, LT .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 2001, 130 (03) :437-460
[67]  
Yura T, 2000, BACTERIAL STRESS RESPONSES, P3
[68]   DNA microarray-mediated transcriptional profiling of the Escherichia coli response to hydrogen peroxide [J].
Zheng, M ;
Wang, X ;
Templeton, LJ ;
Smulski, DR ;
LaRossa, RA ;
Storz, G .
JOURNAL OF BACTERIOLOGY, 2001, 183 (15) :4562-4570