Ras-dependent cell cycle commitment during G2 phase

被引：26

作者：

Hitomi, M ^{[1
]}

Stacey, DW ^{[1
]}

机构：

[1] Cleveland Clin Fdn, Lerner Res Inst, Dept Mol Biol, Cleveland, OH 44195 USA

来源：

FEBS LETTERS | 2001年 / 490卷 / 03期

关键词：

restriction point; cell cycle; ras; cyclin D1; single cell technique;

D O I：

10.1016/S0014-5793(01)02115-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Synchronization used to study cell cycle progression may change the characteristics of rapidly proliferating cells. By combining time-lapse, quantitative fluorescent microscopy and microinjection, we have established a method to analyze the cell cycle progression of individual cells without synchronization. This new approach revealed that rapidly growing NIH3T3 cells make a Ras-dependent commitment for completion of the next fell cycle while they are in G2 phase of the preceding cell cycle. Thus, Ras activity during G2 phase induces cyclin D1 expression. This expression continues through the next G1 phase even in the absence of Ras activity and drives cells into S phase. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：123 / 131

页数：9

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