Highly enantioselective reduction of carbonyl compounds using a reductase purified from bakers' yeast

被引：38

作者：

Ema, T ^{[1
]}

Sugiyama, Y ^{[1
]}

Fukumoto, M ^{[1
]}

Moriya, H ^{[1
]}

Cui, JN ^{[1
]}

Sakai, T ^{[1
]}

Utaka, M ^{[1
]}

机构：

[1] Okayama Univ, Fac Engn, Dept Appl Chem, Okayama 700, Japan

来源：

JOURNAL OF ORGANIC CHEMISTRY | 1998年 / 63卷 / 15期

关键词：

D O I：

10.1021/jo980165e

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

An NADPH-dependent reductase that shows reducing activity for 1-chloro-2-hexanone has been purified from bakers' yeast. SDS-PAGE and gel filtration suggested that the purified reductase is a monomeric enzyme with a molecular weight of ca. 37 kDa. Asymmetric reduction of several carbonyl compounds using the purified reductase has been carried out. 1-Chloro-2-hexanone, 1-acetoxy-2-heptanone, methyl acetoacetate, ethyl pyruvate, 1-chloro-2,4-pentanedione, and 2,4-hexanedione were reduced to the corresponding alcohols with high enantiomeric purities (>98% ee). The reductase showed high specificity constants (k(cat)/K-m = 10(3)-10(5) s(-1) M-1) and relatively low Michaelis constants (K-m= 10(-4)-10(-3) M) for all the substrates examined.

引用

页码：4996 / 5000

页数：5

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