Myogenic induction of aligned mesenchymal stem cell sheets by culture on thermally responsive electrospun nanofibers

被引：185

作者：

Dang, Jiyoung M.

Leong, Kam W.

机构：

[1] Duke Univ, Dept Biomed Engn, Durham, NC 27708 USA

[2] Johns Hopkins Univ, Dept Biomed Engn, Baltimore, MD 21205 USA

来源：

ADVANCED MATERIALS | 2007年 / 19卷 / 19期

关键词：

D O I：

10.1002/adma.200602159

中图分类号：

O6 [化学];

学科分类号：

0703 [化学];

摘要：

A thermally reversible culture substrate with a topographically active surface of aligned nanofibers is able to induce cytoskeletal alignment and nucleus elongation. These morphological changes induce myogenic differentiation in stem cells. The differentiated cells can be recovered in sheet form by thermally induced dissolution of the substrate. A surface able to provide topographical cues to create an aligned stem cell sheet, activated to differentiate to a specific lineage, can have a significant impact on engineering of tissue constructs for regenerative medicine applications.

引用

收藏

页码：2775 / +

页数：6

相关论文

共 41 条

[1]

Nanoscale topography of the corneal epithelial basement membrane and Descemet's membrane of the human [J].

Abrams, GA ;

Schaus, SS ;

Goodman, SL ;

Nealey, PF ;

Murphy, CJ .

CORNEA, 2000, 19 (01) :57-64

[2]

Environmental cues to guide stem cell fate decision for tissue engineering applications [J].

Alsberg, Eben ;

von Recum, Horst A. ;

Mahoney, Melissa J. .

EXPERT OPINION ON BIOLOGICAL THERAPY, 2006, 6 (09) :847-866

[3]

Effect of fiber diameter on spreading, proliferation, and differentiation of osteoblastic cells on electrospun poly(lactic acid) substrates [J].

Badami, AS ;

Kreke, MR ;

Thompson, MS ;

Riffle, JS ;

Goldstein, AS .

BIOMATERIALS, 2006, 27 (04) :596-606

[4]

High RhoA activity maintains the undifferentiated mesenchymal cell phenotype, whereas RhoA down-regulation by laminin-2 induces smooth muscle myogenesis [J].

Beqaj, S ;

Jakkaraju, S ;

Mattingly, RR ;

Pan, D ;

Schuger, L .

JOURNAL OF CELL BIOLOGY, 2002, 156 (05) :893-903

[5]

MyoD and the transcriptional control of myogenesis [J].

Berkes, CA ;

Tapscott, SJ .

SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, 2005, 16 (4-5) :585-595

[6]

Electrospun chitosan-based nanofibers and their cellular compatibility [J].

Bhattarai, N ;

Edmondson, D ;

Veiseh, O ;

Matsen, FA ;

Zhang, MQ .

BIOMATERIALS, 2005, 26 (31) :6176-6184

[7]

Endothelial FAK is essential for vascular network stability, cell survival, and lamellipodial formation [J].

Braren, R ;

Hu, HQ ;

Kim, YH ;

Beggs, HE ;

Reichardt, LF ;

Wang, R .

JOURNAL OF CELL BIOLOGY, 2006, 172 (01) :151-162

[8]

Characterizing nanoscale topography of the aortic heart valve basement membrane for tissue engineering heart valve scaffold design [J].

Brody, S ;

Anilkumar, T ;

Liliensiek, S ;

Last, JA ;

Murphy, CJ ;

Pandit, A .

TISSUE ENGINEERING, 2006, 12 (02) :413-421

[9]

Bone regeneration on computer-designed nano-fibrous scaffolds [J].

Chen, VJ ;

Smith, LA ;

Ma, PX .

BIOMATERIALS, 2006, 27 (21) :3973-3979

[10]

Sustained release of proteins from electrospun biodegradable fibers [J].

Chew, SY ;

Wen, J ;

Yim, EKF ;

Leong, KW .

BIOMACROMOLECULES, 2005, 6 (04) :2017-2024

← 1 2 3 4 5 →