Label-free monitoring of multiple biomolecular binding interactions in real-time with diffraction-based sensing

被引：43

作者：

Goh, JB

Loo, RW

Goh, MC

机构：

[1] Univ Toronto, Dept Chem, Toronto, ON M5S 3H6, Canada

[2] Axela Biosensors Inc, Toronto, ON M5G 1V2, Canada

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2005年 / 106卷 / 01期

关键词：

biosensor; diffraction; immunoassay; microcontact printing; multiple analyte; real-time;

D O I：

10.1016/j.snb.2004.08.003

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Diffraction-based sensing is used to monitor in real-time the competitive response of two different capture molecules simultaneously to different solutions of target analyte. These interactions can be observed directly without labeling any of the molecules involved. The technique is demonstrated using a model system consisting of mouse and rabbit IgG as capture molecules and anti-mouse IgG and anti-rabbit IgG as target analytes. © 2004 Elsevier B.V. All rights reserved.

引用

页码：243 / 248

页数：6

共 14 条

[1] Micropatterned polymeric gratings as chemoresponsive volatile organic compound sensors: Implications for analyte detection and identification via diffraction-based sensor arrays [J].