Modular cloning in plant cells

被引：461

作者：

Karimi, M ^{[1
]}

De Meyer, B ^{[1
]}

Hilson, P ^{[1
]}

机构：

[1] State Univ Ghent VIB, Dept Plant Syst Biol, B-9052 Ghent, Belgium

来源：

TRENDS IN PLANT SCIENCE | 2005年 / 10卷 / 03期

关键词：

D O I：

10.1016/j.tplants.2005.01.008

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

New plant genes are being discovered at a rapid pace. Yet, in most cases, their precise function remains elusive. The recent advent of recombinational cloning techniques has significantly improved our ability to investigate gene functions systematically. For example, proteins fused with diverse fluorescent tags can be expressed at will using versatile cloning cassettes. In addition, novel binary T-DNA vectors are now available to assemble multiple DNA fragments simultaneously, which greatly facilitate plant cell and protein engineering.

引用

页码：103 / 105

页数：3

共 15 条

[1] ORFeome cloning and systems biology: Standardized mass production of the parts from the parts-list [J].

Brasch, MA ;

Hartley, JL ;

Vidal, M .

GENOME RESEARCH, 2004, 14 (10B) :2001-2009

[2] Concerted assembly and cloning of multiple DNA segments using in vitro site-specific recombination: Functional analysis of multi-segment expression clones [J].

Cheo, DL ;

Titus, SA ;

Byrd, DRN ;

Hartley, JL ;

Temple, GF ;

Brasch, MA .

GENOME RESEARCH, 2004, 14 (10B) :2111-2120

[3] A gateway cloning vector set for high-throughput functional analysis of genes in planta [J].

Curtis, MD ;

Grossniklaus, U .

PLANT PHYSIOLOGY, 2003, 133 (02) :462-469

[4] A first version of the Caenorhabditis elegans promoterome [J].

Dupuy, D ;

Li, QR ;

Deplancke, B ;

Boxem, M ;

Hao, T ;

Lamesch, P ;

Sequerra, R ;

Bosak, S ;

Doucette-Stamm, L ;

Hope, IA ;

Hill, DE ;

Walhout, AJM ;

Vidal, M .

GENOME RESEARCH, 2004, 14 (10B) :2169-2175

[5] Genome-wide ORFeome cloning and analysis of Arabidopsis transcription factor genes [J].

Gong, W ;

Shen, YP ;

Ma, LG ;

Pan, Y ;

Du, YL ;

Wang, DH ;

Yang, JY ;

Hu, LD ;

Liu, XF ;

Dong, CX ;

Ma, L ;

Chen, YH ;

Yang, XY ;

Gao, Y ;

Zhu, DM ;

Tan, XL ;

Mu, JY ;

Zhang, DB ;

Liu, YL ;

Dinesh-Kumar, SP ;

Li, Y ;

Wang, XP ;

Gu, HY ;

Qu, LJ ;

Bai, SN ;

Lu, YT ;

Li, JY ;

Zhao, JD ;

Zuo, JR ;

Huang, H ;

Deng, XW ;

Zhu, YX .

PLANT PHYSIOLOGY, 2004, 135 (02) :773-782

[6] Plant responses to ethylene gas are mediated by SCF EBF1/EBF2-dependent proteolysis of EIN3 transcription factor [J].

Guo, HW ;

Ecker, JR .

CELL, 2003, 115 (06) :667-677

[7] DNA cloning using in vitro site-specific recombination [J].

Hartley, JL ;

Temple, GF ;

Brasch, MA .

GENOME RESEARCH, 2000, 10 (11) :1788-1795

[8] Constructs and methods for high-throughput gene silencing in plants [J].

Helliwell, C ;

Waterhouse, P .

METHODS, 2003, 30 (04) :289-295

[9] Versatile gene-specific sequence tags for Arabidopsis functional genomics:: Trancript profiling and reverse genetics applications [J].

Hilson, P ;

Allemeersch, J ;

Altmann, T ;

Aubourg, S ;

Avon, A ;

Beynon, J ;

Bhalerao, RP ;

Bitton, F ;

Caboche, M ;

Cannoot, B ;

Chardakov, V ;

Cognet-Holliger, C ;

Colot, V ;

Crowe, M ;

Darimont, C ;

Durinck, S ;

Eickhoff, H ;

de Longevialle, AF ;

Farmer, EE ;

Grant, M ;

Kuiper, MTR ;

Lehrach, H ;

Léon, C ;

Leyva, A ;

Lundeberg, J ;

Lurin, C ;

Moreau, Y ;

Nietfeld, W ;

Paz-Ares, J ;

Reymond, P ;

Rouzé, P ;

Sandberg, G ;

Segura, MD ;

Serizet, C ;

Tabrett, A ;

Taconnat, L ;

Thareau, V ;

Van Hummelen, P ;

Vercruysse, S ;

Vuylsteke, M ;

Weingartner, M ;

Weisbeek, PJ ;

Wirta, V ;

Wittink, FRA ;

Zabeau, M ;

Small, I .

GENOME RESEARCH, 2004, 14 (10B) :2176-2189

[10] European consortia building integrated resources for Arabidopsis functional genomics [J].

Hilson, P ;

Small, I ;

Kuiper, MTR .

CURRENT OPINION IN PLANT BIOLOGY, 2003, 6 (05) :426-429

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