Induction of mucosal IgA by a novel jet delivery technique for HIV-1 DNA

被引：61

作者：

Lundholm, P ^{[1
]}

Asakura, Y

Hinkula, J

Lucht, E

Wahren, B

机构：

[1] Karolinska Inst, Swedish Inst Infect Dis Control, Ctr Microbiol & Tumor Biol, S-17182 Stockholm, Sweden

[2] Huddinge Univ Hosp F82, Karolinska Inst, Dept Immunol Microbiol Pathol & Infect Dis, S-14186 Huddinge, Sweden

来源：

VACCINE | 1999年 / 17卷 / 15-16期

关键词：

DNA vaccine; HIV-1; mucosal immunization; IgA; IgG; B-cell proliferation;

D O I：

10.1016/S0264-410X(98)00404-6

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Novel ways of delivering plasmid DNA to elicit humoral IgA, IgG and cell-mediated immune responses in mice were investigated. Intraoral administration of DNA in the cheek, using a jet immunization technique, elicited the highest IgA mucosal responses, Intranasal immunization gave strong mucosal IgA responses and persistent systemic IgG, Immunoglobulin isotype analysis revealed an IgG1 profile for intramuscular tongue and gene gun immunizations and an IgG,a profile following oral et injection and intranasal application. The route of delivery was of importance for the characteristics and quality of the mucosal immune response following DNA immunization. For DNA vaccine delivery, the intraoral jet injection technique has the advantages of being a simple and rapid way of administering the DNA in solution and of provoking specific mucosal IgA when administered in the mucosal associated lymphoid tissue. (C) 1999 Elsevier Science Ltd. All rights reserved.

引用

页码：2036 / 2042

页数：7

共 18 条

[1] HUMAN DYSTROPHIN EXPRESSION IN MDX MICE AFTER INTRAMUSCULAR INJECTION OF DNA CONSTRUCTS [J].