Microwave-accelerated metal-enhanced fluorescence:: application to detection of genomic and exosporium anthrax DNA in <30 seconds

被引：30

作者：

Aslan, Kadir ^{[1
]}

Zhang, Yongxia ^{[1
]}

Hibbs, Stephen ^{[1
]}

Baillie, Les ^{[1
]}

Previte, Michael J. R. ^{[1
]}

Geddes, Chris D. ^{[1
]}

机构：

[1] Univ Maryland, Inst Biotechnol, Ctr Med Biotechnol, Lab Adv Med Plasmon, Baltimore, MD 21201 USA

来源：

ANALYST | 2007年 / 132卷 / 11期

关键词：

D O I：

10.1039/b707876e

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We describe the ultra-fast and sensitive detection of the gene encoding the protective antigen of Bacillus anthracis the causative agent of anthrax. Our approach employs a highly novel platform technology, Microwave-Accelerated Metal-Enhanced Fluorescence (MAMEF), which combines the use of Metal-Enhanced Fluorescence to enhance assay sensitivity and focused microwave heating to spatially and kinetically accelerate DNA hybridization. Genomic and exosporium target DNA of Bacillus anthracis spores was detected within a minute in the nanograms per microliter concentration range using low-power focused microwave heating. The MAMEF technology was able to distinguish between B. anthracis and B. cereus, a non-virulent close relative. We believe that this study has set the stage and indeed provides an opportunity for the ultra-fast and specific detection of B. anthracis spores with minimal pre-processing steps using a relatively simple but cost-effective technology that could minimize casualties in the event of another anthrax attack.

引用

页码：1130 / 1138

页数：9

共 47 条

[41] Qualification and performance characteristics of a quantitative enzyme-linked immunosorbent assay for human IgG antibodies to anthrax lethal factor antigen [J].