Comparison of preparative reversed phase liquid chromatography and countercurrent chromatography for the kilogram scale purification of crude spinetoram insecticide

被引：51

作者：

DeAmicis, Carl ^{[1
]}

Edwards, Neil A. ^{[2
]}

Giles, Michael B. ^{[2
]}

Harris, Guy H. ^{[3
]}

Hewitson, Peter ^{[4
]}

Janaway, Lee ^{[2
]}

Ignatova, Svetlana ^{[4
]}

机构：

[1] Dow AgroSci LLC, Indianapolis, IN 46268 USA

[2] Dynam Extract Ltd, Slough, Berks, England

[3] Dynam Extract Inc, Monmouth Jct, NJ 08852 USA

[4] Brunel Univ, Adv Bioproc Ctr, Uxbridge UB8 3PH, Middx, England

来源：

JOURNAL OF CHROMATOGRAPHY A | 2011年 / 1218卷 / 36期

基金：

英国生物技术与生命科学研究理事会;

关键词：

Pilot scale; HPCCC; HPLC; Spinetoram; Spinosyns;

D O I：

10.1016/j.chroma.2011.06.073

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Reversed phase HPLC (RP-HPLC) and high performance countercurrent chromatography (HPCCC) were compared for the pilot scale purification of two semi-synthetic spinosyns, spinetoram-J and spinetoram-L, the major components of the commercial insecticide spinetoram. Two, independently performed, 1 kg, purification campaigns were compared. Each method resulted in the isolation of both components at a purity of >97% and yields for spinetoram-J and spinetoram-L of >93% and >= 63% of theoretical, respectively. The HPCCC process produced a 2-fold higher throughput and consumed approximately 70% less solvent than preparative scale RP-HPLC, the volume of product containing fractions from HPCCC amounted to 7% of that produced by HPLC and so required much less post-run processing. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：6122 / 6127

页数：6

共 10 条

[1] Rapid purification and scale-up of honokiol and magnolol using high-capacity high-speed counter-current chromatography [J].