Lipopolysaccharide-binding protein mediates CD14-independent intercalation of lipopolysaccharide into phospholipid membranes

被引:106
作者
Schromm, AB
Brandenburg, K
Rietschel, ET
Flad, HD
Carroll, SF
Seydel, U
机构
[1] RES CTR BORSTEL,CTR MED & BIOSCI,D-23845 BORSTEL,GERMANY
[2] XOMA CORP,BERKELEY,CA 94710
关键词
LBP; endotoxin; bactericidal/permeability-increasing protein; nonspecific intercalation; phospholipid membrane;
D O I
10.1016/S0014-5793(96)01338-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Lipopolysaccharides (LPS, endotoxin) stimulate mononuclear cells to release cytokines which initiate endotoxic effects, Interaction of LPS at low concentrations with target cells is CD14-dependent whereas at high LPS concentrations it is CD14-independent, Here, we demonstrate by resonance energy transfer (RET) technique that nonspecific, CD14-independent intercalation of LPS into membrane systems can be mediated by lipopolysaccharide-binding protein (LBP), It is proposed that in this pathway, LBP breaks down LPS aggregates, transports the smaller units to and inserts them into the phospholipid cell matrix, We furthermore show that LBP also mediates the intercalation of other negatively charged amphiphilic molecules, We propose a model explaining CD14-independent cell activation at high endotoxin concentrations.
引用
收藏
页码:267 / 271
页数:5
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