Single-Molecule Four-Color FRET

被引：135

作者：

Lee, Jinwoo ^{[1
]}

Lee, Sanghwa ^{[1
]}

Ragunathan, Kaushik

Joo, Chirlmin ^{[3
]}

Ha, Taekjip ^{[2
]}

Hohng, Sungchul ^{[1
]}

机构：

[1] Seoul Natl Univ, Natl Ctr Creat Res Initiat, Dept Biophys & Chem Biol, Dept Phys & Astron, Seoul 151747, South Korea

[2] Univ Illinois, Dept Phys, Ctr Phys Living Cells, Ctr Biophys & Computat Biol,Howard Hughes Med Ins, Urbana, IL 61801 USA

[3] Seoul Natl Univ, Sch Biol Sci, Seoul 151747, South Korea

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2010年 / 49卷 / 51期

基金：

美国国家科学基金会;

关键词：

conformational dynamics; DNA structures; fluorescence; FRET; single-molecule studies; ALTERNATING-LASER EXCITATION; REAL-TIME; ENERGY-TRANSFER; SPECTROSCOPY; DYNAMICS;

D O I：

10.1002/anie.201005402

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Six interfluorophore FRET efficiencies Eij (see scheme) can be determined in real time by a single-molecule four-color FRET technique both in confocal and in total-internal-reflection fluorescence microscopy. This technique was used to probe the correlated motion of the four arms of the Holliday junction, and to assess correlation of RecA-mediated strand exchange events at both ends of a synaptic complex. Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：9922 / 9925

页数：4

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