Quantitative determination of the 3D dipole orientation of single molecules

被引：28

作者：

Débarre, A

Jaffiol, R

Julien, C

Nutarelli, D

Richard, A

Tchénio, P

Chaput, F

Boilot, JP

机构：

[1] CNRS, Aime Cotton Lab, UPR 3321, F-91405 Orsay, France

[2] Ecole Polytech, CNRS, UMR 7643, Grp Chim Solide Phys Mat Condensee, F-91128 Palaiseau, France

来源：

EUROPEAN PHYSICAL JOURNAL D | 2004年 / 28卷 / 01期

关键词：

D O I：

10.1140/epjd/e2003-00295-1

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Recently, different approaches have been implemented to detect single molecules with an optical dipole out of the sample plane. Principles to solve this problem of general interest have been laid but no detailed analysis has been performed to date. This paper is devoted to a quantitative analysis of the dipole orientation of out of plane molecules that we have detected by amplitude and phase masking of the input beam. The accuracy of the orientation is discussed.

引用

页码：67 / 77

页数：11

共 25 条

[1]

Azoulay J, 1999, J MICROSC-OXFORD, V194, P486, DOI 10.1046/j.1365-2818.1999.00558.x

[2] Quenching and enhancement of single-molecule fluorescence under metallic and dielectric tips [J].

Azoulay, J ;

Débarre, A ;

Richard, A ;

Tchénio, P .

EUROPHYSICS LETTERS, 2000, 51 (04) :374-380

[3]

Azoulay J, 2001, SINGLE MOL, V2, P241, DOI 10.1002/1438-5171(200112)2:4<241::AID-SIMO241>3.0.CO

[4]

2-X

[5] Imaging three-dimensional single molecule orientations [J].

Bartko, AP ;

Dickson, RM .

JOURNAL OF PHYSICAL CHEMISTRY B, 1999, 103 (51) :11237-11241

[6]

Basche T., 1997, SINGLE MOL OPTICAL D

[7] SINGLE MOLECULES OBSERVED BY NEAR-FIELD SCANNING OPTICAL MICROSCOPY [J].

BETZIG, E ;

CHICHESTER, RJ .

SCIENCE, 1993, 262 (5138) :1422-1425

[8] Three-dimensional orientation measurements of symmetric single chromophores using polarization microscopy [J].

Empedocles, SA ;

Neuhauser, R ;

Bawendi, MG .

NATURE, 1999, 399 (6732) :126-130

[9] Fluorescence detection of single molecules near a solution/glass interface - an electrodynamic analysis [J].

Enderlein, J .

CHEMICAL PHYSICS LETTERS, 1999, 308 (3-4) :263-266

[10] Rapid determination of the three-dimensional orientation of single molecules [J].

Fourkas, JT .

OPTICS LETTERS, 2001, 26 (04) :211-213

← 1 2 3 →