Rapid determination of the three-dimensional orientation of single molecules

被引：143

作者：

Fourkas, JT ^{[1
]}

机构：

[1] Boston Coll, Eugene F Merkert Chem Ctr, Chestnut Hill, MA 02467 USA

来源：

OPTICS LETTERS | 2001年 / 26卷 / 04期

关键词：

D O I：

10.1364/OL.26.000211

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

A technique is proposed for determination of the three-dimensional orientation of the transition dipoles of single molecules by use of polarization-sensitive detection of fluorescence through a high-N.A. objective. Molecular orientation can be determined uniquely and rapidly based on the counts from three detectors that are sensitive to different polarizations of light. (C) 2001 Optical Society of America OCIS codes: 180.2520, 260.5430.

引用

页码：211 / 213

页数：3

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